Biodegradation of agave Comiteco bagasse by Pleurotus spp.: a source of cellulases useful in hydrolytic treatment to produce reducing sugars.

Autor: Lagunes-Reyes M; El Colegio de la Frontera Sur, Carr. Antiguo Aeropuerto km 2.5, 30700 Tapachula, Chiapas Mexico., Sánchez JE; El Colegio de la Frontera Sur, Carr. Antiguo Aeropuerto km 2.5, 30700 Tapachula, Chiapas Mexico., Andrade-Gallegos RH; El Colegio de la Frontera Sur, Carr. Antiguo Aeropuerto km 2.5, 30700 Tapachula, Chiapas Mexico., Gutiérrez-Hernández RF; Departamento de Ingeniería Química y Bioquímica, Instituto Tecnológico de Tapachula, Tecnológico Nacional de México, 30700 Tapachula, Chiapas Mexico., Camacho-Morales RL; Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Álvaro Obregón s/n, Nueva, 21100 Mexicali, BC Mexico.
Jazyk: angličtina
Zdroj: 3 Biotech [3 Biotech] 2023 Nov; Vol. 13 (11), pp. 356. Date of Electronic Publication: 2023 Oct 07.
DOI: 10.1007/s13205-023-03783-w
Abstrakt: This study aimed to determine the production parameters of five strains of Pleurotus spp. during their cultivation on agave Comiteco bagasse, as well as the feasibility of using cellulolytic extracts to produce reducing sugars in the same bagasse. After cultivation, the basidiome production parameters varied between 41.2 and 65.7% (biological efficiency), 0.17 and 0.30 (yield), 0.60 and 0.90% (production rate), 16.4 and 41.1% (Bioconversion) and 9.4 and 21.3 g (mean mushroom weight). At day 15 of growth, P. djamor showed the highest β-glucosidase activity (43.95 ± 4.5 IU/g); on day 33. The same strain had the highest endoglucanase activity (21.12 ± 0.5 IU/ml). Both extracts were partially purified, and the kinetic parameters V max and K m were estimated (20.83 µmole/ml sec and 232.01 µmole/ml for β-glucosidase and 685.01 µmole/ml sec and 1,240.34 µmole/ml for endoglucanase). In the enzymatic hydrolysis assay, the highest concentration of reducing sugars (43.13 ± 1.09 g/L; 0.21 g/g bagasse) was obtained by a mixture of the two partially purified extracts acting synergistically after 48 h and with a pH adjustment. The results suggest that the use of agave Comiteco bagasse for cultivating edible mushrooms while obtaining cellulolytic extracts is an alternative treatment for waste reduction and valorization of agro-industrial by-products.
Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest.
(© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)
Databáze: MEDLINE
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