An artificially split class 3 intein.
| Autor: | Ariagno TM; Department of Biological Sciences, Murray State University, Murray, Kentucky, United States., Smetana JS; Department of Biological Sciences, Murray State University, Murray, Kentucky, United States., Lennon CW; Department of Biological Sciences, Murray State University, Murray, Kentucky, United States. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2023 Sep 22; Vol. 2023. Date of Electronic Publication: 2023 Sep 22 (Print Publication: 2023). |
| DOI: | 10.17912/micropub.biology.000977 |
| Abstrakt: | Inteins excise themselves from precursor polypeptides through protein splicing, joining N- and C-exteins with a peptide bond. Split inteins are expressed as separate polypeptides that undergo protein trans splicing (PTS). Here, we demonstrate PTS can be achieved using an artificially split class 3 intein. Because class 3 inteins use an internal initiating nucleophile near the C-extein junction, rather than the first residue of the intein, both catalytic nucleophiles are present on a single polypeptide. This results in a compact arrangement of catalytic nucleophiles for PTS compared to the standard arrangement for split class 1 inteins. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2023 by the authors.) |
| Databáze: | MEDLINE |
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