Bioactive profiles of edible vegetable oils determined using 10D hyphenated comprehensive high-performance thin-layer chromatography (HPTLC×HPTLC) with on-surface metabolism (nanoGIT) and planar bioassays.

Autor:	Müller I; Institute of Nutritional Science, Chair of Food Science, as well as Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany., Gulde A; Institute of Nutritional Science, Chair of Food Science, as well as Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany., Morlock GE; Institute of Nutritional Science, Chair of Food Science, as well as Interdisciplinary Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Giessen, Germany.; Center for Sustainable Food Systems, Justus Liebig University Giessen, Giessen, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in nutrition [Front Nutr] 2023 Sep 22; Vol. 10, pp. 1227546. Date of Electronic Publication: 2023 Sep 22 (Print Publication: 2023).
DOI:	10.3389/fnut.2023.1227546
Abstrakt:	Introduction: Vegetable oils rich in unsaturated fatty acids are assumed to be safe and even healthy for consumers though lipid compositions of foods vary naturally and are complex considering the wealth of minor compounds down to the trace level. Methods: The developed comprehensive high-performance thin-layer chromatography (HPTLC×HPTLC) method including the on-surface metabolization (nanoGIT) and bioassay detection combined all steps on the same planar surface. The pancreatic lipolysis (intestinal phase) experiment and the subsequent analysis of the fatty acid composition including its effect-directed detection using a planar bioassay was performed without elaborate sample preparation or fractionation to ensure sample integrity. Thus, no sample part was lost, and the whole sample was studied on a single surface regarding all aspects. This made the methodology as well as technology miniaturized, lean, all-in-one, and very sustainable. Results and Discussion: To prioritize important active compounds including their metabolism products in the complex oil samples, the nanoGIT method was used to examine the pancreatic lipolysis of nine different vegetable oils commonly used in the kitchen and food industry, e.g., canola oil, flaxseed oil, hemp oil, walnut oil, soybean oil, sunflower oil, olive oil, coconut oil, and palm oil. The digested oils revealed antibacterial and genotoxic effects, which were assigned to fatty acids and oxidized species via high-resolution tandem mass spectrometry (HRMS/MS). This finding reinforces the importance of adding powerful techniques to current analytical tools. The 10D hyphenated nanoGIT-HPTLC×HPTLC-Vis/FLD-bioassay-heart cut-RP-HPLC-DAD-HESI-HRMS/MS has the potential to detect any potential hazard due to digestion/metabolism, improving food safety and understanding on the impact of complex samples. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer PR declared a past co-authorship with the author GM to the handling editor. (Copyright © 2023 Müller, Gulde and Morlock.)
Databáze:	MEDLINE
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