Improvement of triterpenoid production in mycelia of Antrodia camphorata through mutagenesis breeding and amelioration of CCl 4 -induced liver injury in mice.
| Autor: | Wang HJ; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Cui C; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Gong XM; Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China., Wang S; Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China., Li CX; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China.; School of Life Science, Bengbu Medical College, Bengbu, 233030, China., Guo H; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Wang YL; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Huang YD; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Jiang JL; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Luo XM; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Miao JH; Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China., Liu TQ; Jiangbin Hospital of Guangxi Zhuang Autonomous Region, Nanning, 530021, China., Zhao S; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China., Feng JX; State Key Laboratory for Conservation and Utilization of Subtropical Agro Bioresources, Guangxi Research Center for Microbial and Enzyme Engineering Technology, College of Life Science and Technology, Guangxi University, Nanning, 530004, China. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2023 Aug 30; Vol. 9 (9), pp. e19621. Date of Electronic Publication: 2023 Aug 30 (Print Publication: 2023). |
| DOI: | 10.1016/j.heliyon.2023.e19621 |
| Abstrakt: | Due to the scarcity of wild fruiting bodies, submerged fermentation of the medicinal fungus Antrodia camphorata is attracting much attention, but the production of bioactive triterpenoids is low. Therefore, there is an urgent need to improve the triterpenoid yield of submerged fermentation. Here, the A. camphorata mutant E3-64 was generated from strain AC16101 through random mutagenesis breeding, producing 172.8 mg triterpenoid per gram of dry mycelia. Further optimization of culture parameters resulted in a yield of 255.5 mg/g dry mycelia (i.e., an additional >1.4-fold increase), which is the highest reported yield thus far. Notably, mutant E3-64 produced 94% and 178% more of the triterpenoid components antcin A and antcamphin A, respectively, while it produced 52% and 15% less antcin B and G, respectively. Mutant E3-64 showed increased expression of key genes involved in triterpenoid biosynthesis, as well as different genome-wide single-nucleotide polymorphisms as compared with AC16101. Triterpenoids of the E3-64 mycelia exhibited remarkably protective activity against acute CCl Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|