Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites.

Autor:	Atkinson L; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK., Lee JC; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK., Lennon A; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK., Shah D; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK., Storey N; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK., Morfopoulou S; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.; Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, UK., Harris KA; Royal London Hospital, Barts Health NHS Trust, Department of Virology, London, UK., Breuer J; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.; Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, UK., Brown JR; Great Ormond Street Hospital for Children NHS Foundation Trust, Department of Microbiology, Virology and Infection Control, London, UK.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2023 Sep 09; Vol. 9 (9), pp. e19854. Date of Electronic Publication: 2023 Sep 09 (Print Publication: 2023).
DOI:	10.1016/j.heliyon.2023.e19854
Abstrakt:	Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 Published by Elsevier Ltd.)
Databáze:	MEDLINE
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