Survey of white-footed mice in Connecticut, USA reveals low SARS-CoV-2 seroprevalence and infection with divergent betacoronaviruses.

Autor:	Earnest R; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Hahn AM; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Feriancek NM; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Brandt M; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Filler RB; Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06520, USA.; Department of Immunobiology, Yale School of Medicine, New Haven, CT 06520, USA., Zhao Z; Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06520, USA.; Department of Immunobiology, Yale School of Medicine, New Haven, CT 06520, USA., Breban MI; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Vogels CBF; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Chen NFG; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Koch RT; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Porzucek AJ; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Sodeinde A; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Garbiel A; Department of Environmental Science and Forestry, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Keanna C; Department of Environmental Science and Forestry, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Litwak H; Department of Environmental Science and Forestry, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Stuber HR; Department of Entomology, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Cantoni JL; Department of Entomology, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Pitzer VE; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Olarte Castillo XA; Department of Microbiology and Immunology, Cornell University College of Veterinary Medicine, Ithaca, NY 14853., Goodman LB; Department of Public & Ecosystem Health, Cornell University College of Veterinary Medicine, Ithaca, NY 14853., Wilen CB; Department of Laboratory Medicine, Yale School of Medicine, New Haven, CT 06520, USA.; Department of Immunobiology, Yale School of Medicine, New Haven, CT 06520, USA., Linske MA; Department of Entomology, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Williams SC; Department of Environmental Science and Forestry, The Connecticut Agricultural Experiment Station, New Haven, CT 06511, USA., Grubaugh ND; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA.; Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06510, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Sep 27. Date of Electronic Publication: 2023 Sep 27.
DOI:	10.1101/2023.09.22.559030
Abstrakt:	Diverse mammalian species display susceptibility to and infection with SARS-CoV-2. Potential SARS-CoV-2 spillback into rodents is understudied despite their host role for numerous zoonoses and human proximity. We assessed exposure and infection among white-footed mice ( Peromyscus leucopus ) in Connecticut, USA. We observed 1% (6/540) wild-type neutralizing antibody seroprevalence among 2020-2022 residential mice with no cross-neutralization of variants. We detected no SARS-CoV-2 infections via RT-qPCR, but identified non-SARS-CoV-2 betacoronavirus infections via pan-coronavirus PCR among 1% (5/468) of residential mice. Sequencing revealed two divergent betacoronaviruses, preliminarily named Peromyscus coronavirus-1 and - 2 . Both belong to the Betacoronavirus 1 species and are ~90% identical to the closest known relative, Porcine hemagglutinating encephalomyelitis virus . Low SARS-CoV-2 seroprevalence suggests white-footed mice may not be sufficiently susceptible or exposed to SARS-CoV-2 to present a long-term human health risk. However, the discovery of divergent, non-SARS-CoV-2 betacoronaviruses expands the diversity of known rodent coronaviruses and further investigation is required to understand their transmission extent.
Databáze:	MEDLINE
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