Analysis of nasopharyngeal microbiome patterns in Zambian infants with fatal acute febrile illness.

Autor:	Odom AR; Bioinformatics Program, Boston University, Boston, MA, 02118, USA., McClintock J; Division of Infectious Disease, Center for Data Science, Rutgers New Jersey Medical School, Newark, NJ, 07103, USA., Gill CJ; Department of Global Health, Boston University School of Public Health, Boston, MA, 02118, USA., Pieciak R; Department of Global Health, Boston University School of Public Health, Boston, MA, 02118, USA., Ismail A; Sequencing Core Facility, National Institute for Communicable Diseases of the National Health Laboratory Service, 2131 Johannesburg, South Africa.; Department of Biochemistry and Microbiology, University of Venda, Thohoyandou 0950, South Africa., MacLeod WB; Department of Global Health, Boston University School of Public Health, Boston, MA, 02118, USA., Johnson WE; Division of Infectious Disease, Center for Data Science, Rutgers New Jersey Medical School, Newark, NJ, 07103, USA., Lapidot R; Pediatric Infectious Diseases, Boston Medical Center, Boston, MA, 02118, USA.; Department of Pediatrics, Boston University School of Medicine, Boston, MA, 02118, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Sep 28. Date of Electronic Publication: 2023 Sep 28.
DOI:	10.1101/2023.09.27.559805
Abstrakt:	Introduction: Associative connections have previously been identified between nasopharyngeal infections and infant mortality. The nasopharyngeal microbiome may potentially influence the severity of these infections. Methods: We conducted an analysis of a longitudinal prospective cohort study of 1,981 infants who underwent nasopharyngeal sampling from 1 week through 14 weeks of age at 2-3-week intervals. In all, 27 microbiome samples from 9 of the infants in the cohort who developed fatal acute febrile illness (fAFI) were analyzed in pooled comparisons with 69 samples from 10 healthy comparator infants. We completed 16S rRNA amplicon gene sequencing all infant NP samples and characterized the maturation of the infant NP microbiome among the fAFI(+) and fAFI(-) infant cohorts. Results: Beta diversity measures of fAFI(-) infants were markedly higher than those of fAFI(+) infants. The fAFI(+) infant NP microbiome was marked by higher abundances of Escherichia, Pseudomonas, Leuconostoc , and Weissella , with low relative presence of Alkalibacterium, Dolosigranulum, Moraxella , and Streptococcus . Conclusions: Our results suggest that nasopharyngeal microbiome dysbiosis precedes fAFI in young infants. Early dysbiosis, involving microbes such as Escherichia , may play a role in the causal pathway leading to fAFI or could be a marker of other pathogenic forces that directly lead to fAFI. Competing Interests: Conflicts of Interest The authors declare that they have no conflicts of interest.
Databáze:	MEDLINE
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