Cell free extract-mediated biogenic synthesis of ZnONPs and their application with kanamycin as a bactericidal combination.

Autor: Saleha A; Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, MS, 444 602, India., Shende SS; Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, MS, 444 602, India. sudhirsshende13884@gmail.com.; Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia. sudhirsshende13884@gmail.com., Ingle P; Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, MS, 444 602, India., Rai M; Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, MS, 444 602, India.; Department of Microbiology, Nicolaus Copernicus University, 87-100, Torun, Poland., Minkina TM; Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, 344090, Russia., Gade A; Nanobiotechnology Laboratory, Department of Biotechnology, Sant Gadge Baba Amravati University, Amravati, MS, 444 602, India. aniketgade@sgbau.ac.in.; Department of Microbiology, Nicolaus Copernicus University, 87-100, Torun, Poland. aniketgade@sgbau.ac.in.; Department of Biological Sciences and Biotechnology, Institute of Chemical Technology, Mumbai, Maharashtra, 400019, India. aniketgade@sgbau.ac.in.
Jazyk: angličtina
Zdroj: World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2023 Oct 09; Vol. 39 (12), pp. 334. Date of Electronic Publication: 2023 Oct 09.
DOI: 10.1007/s11274-023-03777-z
Abstrakt: Antimicrobial resistance (AMR) is a main public health issue and a challenge for the scientific community all over the globe. Hence, there is a burning need to build new bactericides that resist the AMR. The ZnONPs were produced by cell free extract of mint (Mentha piperita L.) leaves. Antibiotics that are ineffective against resistant bacteria like Escherichia coli and Staphylococcus aureus were treated. The antibiotics were first screened, and then antibacterial activity was checked by disk diffusion, and MIC of Mp-ZnONPs individually and using Kanamycin (KAN) were determined against these pathogens by broth microdilution method. The synergism between Mp-ZnONPs and KAN was confirmed by checkerboard assay. The MIC showed robust antibacterial activity against the tested pathogens. The combination of KAN and Mp-ZnONPs reduces the MIC of KAN as it efficiently inhibits E. coli's growth, and KAN significantly enhances the antibacterial activity of Mp-ZnONPs. Taken together, Mp-ZnONPs have strong antimicrobial activity, and KAN significantly improves it against the tested pathogens, which would offer an effective, novel, and benign therapeutic methodology to regulate the incidence. The combination of Mp-ZnONPs and KAN would lead to the development of novel bactericides, that could be used in the formulation of pharmaceutical products.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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