Different airborne particulates trigger distinct immune pathways leading to peanut allergy in a mouse model.

Autor: Immormino RM; Department of Pediatrics, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Smeekens JM; Department of Pediatrics, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.; UNC Food Allergy Initiative, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA., Mathai PI; Department of Pediatrics, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Clough KM; University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA., Nguyen JT; University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA., Ghio AJ; Human Studies Facility, United States Environmental Protection Agency, Chapel Hill, North Carolina, USA., Cook DN; Division of Intramural Research, Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, NIH, North Carolina, USA., Kulis MD; Department of Pediatrics, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.; UNC Food Allergy Initiative, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA., Moran TP; Department of Pediatrics, The University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.; Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Jazyk: angličtina
Zdroj: Allergy [Allergy] 2024 Feb; Vol. 79 (2), pp. 432-444. Date of Electronic Publication: 2023 Oct 07.
DOI: 10.1111/all.15908
Abstrakt: Background: Environmental exposure to peanut through non-oral routes is a risk factor for peanut allergy. Early-life exposure to air pollutants, including particulate matter (PM), is associated with sensitization to foods through unknown mechanisms. We investigated whether PM promotes sensitization to environmental peanut and the development of peanut allergy in a mouse model.
Methods: C57BL/6J mice were co-exposed to peanut and either urban particulate matter (UPM) or diesel exhaust particles (DEP) via the airways and assessed for peanut sensitization and development of anaphylaxis following peanut challenge. Peanut-specific CD4 + T helper (Th) cell responses were characterized by flow cytometry and Th cytokine production. Mice lacking select innate immune signaling genes were used to study mechanisms of PM-induced peanut allergy.
Results: Airway co-exposure to peanut and either UPM- or DEP-induced systemic sensitization to peanut and anaphylaxis following peanut challenge. Exposure to UPM or DEP triggered activation and migration of lung dendritic cells to draining lymph nodes and induction of peanut-specific CD4 + Th cells. UPM- and DEP-induced distinct Th responses, but both stimulated expansion of T follicular helper (Tfh) cells essential for peanut allergy development. MyD88 signaling was critical for UPM- and DEP-induced peanut allergy, whereas TLR4 signaling was dispensable. DEP-induced peanut allergy and Tfh-cell differentiation depended on IL-1 but not IL-33 signaling, whereas neither cytokine alone was necessary for UPM-mediated sensitization.
Conclusion: Environmental co-exposure to peanut and PM induces peanut-specific Tfh cells and peanut allergy in mice.
(© 2023 European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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