Enhancing bovine tuberculosis screening at Dhaka city in Bangladesh: Integrating gamma interferon blood test as ancillary testing with tuberculin skin test.

Autor: Islam R; Department of Microbiology, Jagannath University, Dhaka 1100, Bangladesh. Electronic address: shantorobiul@gmail.com., Islam SS; Department of Livestock Services, Krishi Khamar Sarak, Farmgate, Dhaka 1215, Bangladesh; Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh., Rumi TB; International Centre for Diarrhoeal Disease and Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1215, Bangladesh., Mia Z; Department of Microbiology, Jagannath University, Dhaka 1100, Bangladesh., Rahim Z; International Centre for Diarrhoeal Disease and Research, Bangladesh (icddr,b), Mohakhali, Dhaka 1215, Bangladesh.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2023 Oct; Vol. 264, pp. 110659. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1016/j.vetimm.2023.110659
Abstrakt: Tuberculin skin test (TST) is the standard method for screening of bovine tuberculosis (bTB). However, gamma interferon blood test has been introduced in the bTB control program as an ancillary testing with TST in many countries of the world. The objective of this study was to recommend this screening test as an ancillary testing with TST for field application in Bangladesh. In this study 577 cattle of different age, sex and breeds from twenty nine (29) cattle herds were examined to determine skin response against bTB through single intradermal comparative tuberculin test (SICTT) that comprised of positive (n = 81), inconclusive (n = 44) and negative (n = 452) animals. Of which 74 animals that included positive (n = 63), inconclusive (n = 8) and negative (n = 3) animals were taken under this study. Blood samples were collected in heparinized tube and stimulated overnight with bovine and avian purified protein derivatives (PPDs) for the secretion of gamma interferon, and measured via sandwich ELISA. Cohen's kappa statistics was performed for the evaluation of agreement between the two tests. The agreement obtained between two tests was fair (Kappa agreement, K = 24.0%, 95% CI = 16.9-30.5%, P = 0.037). Of positive (n = 63), inconclusive (n = 8) and negative (n = 3) status of animals at SICTT, 82.54% (n = 52), 62.50% (n = 5), and 33.33% (n = 1) were found to be bTB positive respectively through this ancillary test. This test notably corroborates to TST result. A considerable number of inconclusive TB status animals were found to be positive through this gamma interferon assay. Therefore, this test could be used as an ancillary test with TST to maximize the proportion of bTB estimation in the infected cattle herd for early detection of zoonotic tuberculosis in Bangladesh before transmission at the animal-human interface.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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