In vitro evaluation of the potential immunosuppressive effect of panobinostat on cultured lymphocytes retrieved from childhood systemic lupus erythematosus patients.

Autor:	Yassin NA; Department of Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Abdelsalam M; Immunology Unit, Department of Clinical Pathology, Faculty of Medicine, Mansura University, Egypt.; Laboratory Department, Faculty of Allied Health Science, Badr University, Cairo, Egypt., El-Sabbagh AM; Department of Medical Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Morsy AI; Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Haleem AA; Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt., El-Shenbaby I; Department of Pharmacology, Faculty of Medicine, Mansoura University, Mansoura, Egypt., El Wakeel GA; Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt., Hammad A; Pediatric Nephrology Unit, Mansoura University Children's Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Hamdy N; Pediatric Nephrology Unit, Mansoura University Children's Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Abd-El Ghafaar DM; Department of Rheumatology, Faculty of Medicine, Mansoura University, Egypt., Elmarghany EB; Department of Rheumatology, Faculty of Medicine, Mansoura University, Egypt., Korkor MS; Pediatric Nephrology Unit, Mansoura University Children's Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Eid R; Pediatric Nephrology Unit, Mansoura University Children's Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Jazyk:	angličtina
Zdroj:	The Egyptian journal of immunology [Egypt J Immunol] 2023 Oct; Vol. 30 (4), pp. 145-154.
Abstrakt:	Although many drugs are available for childhood systemic lupus erythematosus (SLE) treatment, the adverse effects and poor response in some cases make it crucial to find new drugs targeting various pathways in disease pathogenesis to improve overall outcomes. This study aimed to (i) investigate the effect of Panobinostat on cultured lymphocytes obtained from children with active SLE and (ii) to compare that effect with standard drugs used in SLE, such as Prednisone and hydroxychloroquine. The study included 24 SLE active patients, divided into four equal groups. Lymphocytes were isolated from blood samples of the study patients. According to the study group, cells were treated with either Panobinostat, Prednisolone, hydroxychloroquine, or not treated (control group). After cell culture, the response of lymphocytes upon drug treatment was analyzed in terms of the production of anti-dsDNA antibodies and levels of apoptosis as detected by flow cytometry using annexin V and propidium iodide (PI) staining. The Panobinostat group showed a significant decrease in the viable cell count (p < 0.001). Both Prednisone and hydroxychloroquine decreased anti-dsDNA expression more than the Panobinostat and control groups (p < 0.001 for both). PI was higher in the Prednisone group, and Annexin V was higher in the Panobinostat group compared to other groups; however, their increase did not reach statistically significant levels (p= 0.12 and 0.85, respectively). This is the first study of the Panobinostat effect on cultured lymphocytes of SLE. In conclusion, Panobinostat could be a prospective treatment for B-cell-driven autoimmune diseases such as SLE. However, its effect on autoantibodies levels and different clinical features of SLE still need a thorough evaluation. (Copyright© by the Egyptian Association of Immunologists.)
Databáze:	MEDLINE
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