Preparation of human primary macrophages to study the polarization from monocyte-derived macrophages to pro- or anti-inflammatory macrophages at biomaterial interface in vitro .

Autor:	Parisi L; Laboratory for Oral Molecular Biology, Department of Orthodontics and Dentofacial Orthopedics, University of Bern, Bern, Switzerland., Bianchi MG; Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy.; Microbiome Research Hub, Università di Parma, Parma, Italy., Ghezzi B; Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy.; Centro Universitario di Odontoiatria, Università di Parma, Parma, Italy.; IMEM-CNR, Parma, Italy., Maurizi E; Centro Universitario di Odontoiatria, Università di Parma, Parma, Italy.; Centre for Regenerative Medicine 'S.Ferrari', University of Modena and Reggio Emilia, Modena, Italy., Macaluso GM; Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy.; Centro Universitario di Odontoiatria, Università di Parma, Parma, Italy.; IMEM-CNR, Parma, Italy., Bussolati O; Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy.; Microbiome Research Hub, Università di Parma, Parma, Italy., Lumetti S; Dipartimento di Medicina e Chirurgia, Università di Parma, Parma, Italy.; Centro Universitario di Odontoiatria, Università di Parma, Parma, Italy.; IMEM-CNR, Parma, Italy.
Jazyk:	angličtina
Zdroj:	Journal of dental sciences [J Dent Sci] 2023 Oct; Vol. 18 (4), pp. 1630-1637. Date of Electronic Publication: 2023 Feb 13.
DOI:	10.1016/j.jds.2023.01.020
Abstrakt:	Background/purpose: Testing of dental materials when in contact with innate immune cells has been so far hindered by the lack of proper in vitro models. Human primary monocyte-derived macrophages (MDMs) would be an excellent option to this aim. However, the inability to detach them from the tissue culture plates contrast the possibility to culture them on biomaterials. The goal of the present work is to present and validate an innovative protocol to obtain MDMs from peripheral blood monocytes, and to reseed them in contact with biomaterials without altering their viability and phenotype. Materials and Methods: We differentiated MDMs on ultra-low attachment tissue culture plastics and recovered them with specific detachment solution in order to be reseeded on a secondary substrate. Therefore, using biological assays (RT-PCR, Western blot, and immunofluorescence) we compared their phenotype to MDMs differentiated on standard culture plates. Results: Transferred MDMs keep their differentiated M0 resting state, as well as the ability to be polarized into M1 (pro-inflammatory) or M2 (anti-inflammatory) macrophages. Conclusion: These data provide the dental material research community the unprecedented possibility to investigate the immunomodulatory properties of biomaterials for dental application. Competing Interests: The authors have no conflicts of interest relevant to this article. (© 2023 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.)
Databáze:	MEDLINE
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