| Autor: |
Masciotti V; CNR-IOM, AREA Science Park, Basovizza Trieste I-34149, Italy.; PhD Course in Nanotechnology, University of Trieste, Trieste I-34127, Italy., Piantanida L; CNR-IOM, AREA Science Park, Basovizza Trieste I-34149, Italy., Naumenko D; CNR-IOM, AREA Science Park, Basovizza Trieste I-34149, Italy.; Institute for Physics of Semiconductors, National Academy of Sciences of Ukraine, Kyiv 03028, Ukraine., Amenitsch H; Institute of Inorganic Chemistry, Graz University of Technology, Graz A-8010, Austria., Fanetti M; Materials Research Laboratory, University of Nova Gorica, Nova Gorica SI-5000, Slovenia., Valant M; Materials Research Laboratory, University of Nova Gorica, Nova Gorica SI-5000, Slovenia.; Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu 610054, China., Lei D; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley CA 94720, USA.; School of Physical Science and Technology, Electron Microscopy Center of LZU, Lanzhou University, Lanzhou 730000, China., Ren G; The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley CA 94720, USA., Lazzarino M; CNR-IOM, AREA Science Park, Basovizza Trieste I-34149, Italy. |
| Abstrakt: |
DNA origami is a promising technology for its reproducibility, flexibility, scalability and biocompatibility. Among the several potential applications, DNA origami has been proposed as a tool for drug delivery and as a contrast agent, since a conformational change upon specific target interaction may be used to release a drug or produce a physical signal, respectively. However, its conformation should be robust with respect to the properties of the medium in which either the recognition or the read-out take place, such as pressure, viscosity and any other unspecific interaction other than the desired target recognition. Here we report on the read-out robustness of a tetragonal DNA-origami/gold-nanoparticle hybrid structure able to change its configuration, which is transduced in a change of its plasmonic properties, upon interaction with a specific DNA target. We investigated its response when analyzed in three different media: aqueous solution, solid support and viscous gel. We show that, once a conformational variation is produced, it remains unaffected by the subsequent physical interactions with the environment. |
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