Characterization and application of tannase and gallic acid produced by co-fungi of Aspergillus niger and Trichoderma viride utilizing agro-residues substrates.

Autor: Ahmed AI; Department of Agricultural Microbiology, Faculty of Agriculture, Beni-Suef University, Beni-Suef, Egypt., Abou-Taleb KAA; Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Hadayek Shubra, Cairo, 11241, Egypt., Abd-Elhalim BT; Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Hadayek Shubra, Cairo, 11241, Egypt. basma.talaat@agr.asu.edu.eg.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Oct 05; Vol. 13 (1), pp. 16755. Date of Electronic Publication: 2023 Oct 05.
DOI: 10.1038/s41598-023-43955-5
Abstrakt: Bioconversion using fungi, as natural factory of many applicable bioactive compounds, as enzymes utilizing agro-residue substrates as a solid, abundant, low-cost growth and enzyme production media. This study characterized and applied a tannase enzyme (308 U/mg) from Aspergillus niger A8 + Trichoderma viride co-cultures utilizing pomegranate peels. The partially purified enzyme showed maximal relative activity at 37-65 °C for 10 min and kinetics of thermal inactivation energy at a high point at 60 °C for 0.040/min. The half-life was 37 °C for 58.6 min, temperature coefficient Q 10 of tannase was maximal for 1.38 between 40 and 50 °C, and the activation energy was 17.42 kJ/mol. The enzyme activity peaked in the pH range of 4-8, and the maximum relative activity (100.6%) for tannase was achieved at pH 6. The K m and V max values for purified enzymes using tannic acid were 7.3 mg/mL and 3333.33 U/mL, respectively. The enzyme reduced the total tannin content in all tannin-rich substrates after 12h. The gallic acid (GA) had total phenols of 77.75 ppm and antioxidant activity of 82.91%. It was observed that the GA as antimicrobial influencer exhibited the largest inhibitory zone diameter (IZD) of 31 ± 1.0 mm against Pseudomonas aeruginosa ATCC27853. The GA minimum inhibitory concentration value was ranged from 7770.0-121.41 µg/mL. The obtained GA showed a bactericidal effect against all bacterial strains except Shigella sonnei DSM5570 and Salmonella typhi DSM17058, which showed bacteriostatic behavior.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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