Metabolomics analysis of the metabolic effects of citric acid on Issatchenkia terricola WJL-G4.

Autor: Mei J; College of Life Science, Northeast Forestry University, No. 26 Hexing St., Harbin, Heilongjiang 150040, China., Liu X; Shanghai Urban Construction Vocational College, No. 2080 Nanting St., Shanghai 201415, China., Liu X; College of Life Science, Northeast Forestry University, No. 26 Hexing St., Harbin, Heilongjiang 150040, China., Bao Y; College of Life Science, Northeast Forestry University, No. 26 Hexing St., Harbin, Heilongjiang 150040, China; Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province, No. 26 Hexing St., Harbin, Heilongjiang 150040, China., Luo T; State Key Laboratory of Food Science and Technology, Nanchang University, No. 999 Xuefu St., Nanchang, Jiangxi 330047, China., Wang J; College of Life Science, Northeast Forestry University, No. 26 Hexing St., Harbin, Heilongjiang 150040, China; Key Laboratory of Forest Food Resources Utilization of Heilongjiang Province, No. 26 Hexing St., Harbin, Heilongjiang 150040, China. Electronic address: wangjinling@nefu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of bioscience and bioengineering [J Biosci Bioeng] 2023 Dec; Vol. 136 (6), pp. 452-461. Date of Electronic Publication: 2023 Oct 03.
DOI: 10.1016/j.jbiosc.2023.09.006
Abstrakt: In current research, yeast species Issatchenkia terricola WJL-G4 was shown to be capable of degrading citric acid, especially in the processing of fruit juice and wine. I. terricola WJL-G4 was able to use citric acid as a carbon source, but the metabolic effects of citric acid on yeast remained unclear. In this study, the metabolic effects of citric acid on I. terricola WJL-G4 were studied using liquid chromatography-mass spectrometry metabolomics technology, with glucose treatment as the control. Results showed that organic acid contents related to the extracellular tricarboxylic acid cycle (TCA) varied greatly. The metabolomics results indicated that I. terricola WJL-G4 might metabolize citric acid through the TCA pathway, and the glycolysis pathway might be inhibited; however, gluconeogenesis proceeded normally during citric acid treatment. Some fatty acids and phospholipids, along with the metabolic pathways of amino acids, vitamins, purines and nicotinamide in I. terricola WJL-G4 were also affected by the citric acid treatment. This work provided a theoretical basis for further study of the mechanism of yeast metabolism of citric acid.
(Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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