First study on repeatable culture of primordial germ cells from various embryonic regions with giant feeder cells in Japanese quail (Coturnix japonica).

Autor: Sritabtim K; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand., Prukudom S; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand; Department of Anatomy, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand., Piyasanti Y; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand., Chaipipat S; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand; Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom, Thailand; Center of Excellence on Agricultural Biotechnology:(AG-BIO/PERDO-CHE), Bangkok, Thailand., Kuwana T; Shiga University of Medical Science, Shiga, Japan., Jurutha J; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand., Sinsiri R; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand., Tirawattanawanich C; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand., Siripattarapravat K; Center for Veterinary Diagnostic Laboratory - Bangkhen, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand; Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom, Thailand; Center of Excellence on Agricultural Biotechnology:(AG-BIO/PERDO-CHE), Bangkok, Thailand; Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand. Electronic address: kannika.si@ku.th.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Jan 01; Vol. 213, pp. 43-51. Date of Electronic Publication: 2023 Sep 25.
DOI: 10.1016/j.theriogenology.2023.09.020
Abstrakt: Japanese quail (JQ, Coturnix japonica) is a farmed animal with a high economic value and has been used extensively as an avian model for research. Germline chimera production based on cryopreserved primordial germ cells (PGCs) is possible for conservation management of quail breeds as successful isolation has been reported of PGCs from their blood and gonads. However, the repeatable cultivation protocol has not been elucidated yet, which has hindered technological development. The current study characterized cultivation of pregonadal PGCs isolated from embryonic parts; embryonic blood (cPGCs), whole embryonic tissues (tPGCs), parts of tail buds (tbPGCs), and a mixture of blood and tail bud tissues (ctbPGCs). The results showed that the cultivation system required the presence of specific embryonic cells to act as a feeder for JQ-PGCs and that such a system facilitated more successful cultivation, as shown by the percentages of isolation and cultivation in tbPGCs (100%, 100%, respectively), tPGCs (60%, 55%, respectively), and ctbPGCs (60%, 30%, respectively), but not in cPGCs (0%) cultured on a mitomycin-treated JQ feeder cell-line. Once the co-culture system had been established, the PGCs could be propagated for at least 5 months. These PGCs expressed germ cell-specific markers (DAZL and CVH) and could colonize embryonic gonads. Conclusively, the isolation of pregonadal PGCs and their long-term cultivation in vitro requires a unique embryonic cell, giant cell feeder, that is indispensable for the proliferation of PGCs. Characterization of cell signaling sustaining a mutual interaction between the PGCs and the specific feeder cells will elucidate a superior environment for in vitro cultivation, as well as support the minimal transfer of used xenobiotics in chimera production.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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