Chromosome-level assembly and annotation of the Xyrichtys novacula (Linnaeus, 1758) genome.

Autor:	Cruz F; Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac 4, 08028 Barcelona, Spain., Gómez-Garrido J; Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac 4, 08028 Barcelona, Spain., Gut M; Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac 4, 08028 Barcelona, Spain., Alioto TS; Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac 4, 08028 Barcelona, Spain., Pons J; Institut Mediterrani d'Estudis Avançats, IMEDEA (UIB-CSIC), C/Miquel Marquès 21, 07190 Esporles, Illes Balears, Spain., Alós J; Institut Mediterrani d'Estudis Avançats, IMEDEA (UIB-CSIC), C/Miquel Marquès 21, 07190 Esporles, Illes Balears, Spain., Barcelo-Serra M; Institut Mediterrani d'Estudis Avançats, IMEDEA (UIB-CSIC), C/Miquel Marquès 21, 07190 Esporles, Illes Balears, Spain.
Jazyk:	angličtina
Zdroj:	DNA research : an international journal for rapid publication of reports on genes and genomes [DNA Res] 2023 Oct 01; Vol. 30 (5).
DOI:	10.1093/dnares/dsad021
Abstrakt:	The pearly razorfish (Xyrichtys novacula), commonly known as raor in the Balearic Islands, is a wrasse within the family Labridae. This fish species has particular biological and socio-cultural characteristics making it an ideal model organism in the fields of behavioural ecology, molecular ecology and conservation biology. In this study, we present the first annotated chromosome-level assembly for this species. Sequencing involved a combination of long reads with Oxford Nanopore Technologies, Illumina paired-end short reads (2 × 151 bp), Hi-C and RNA-seq from different tissues. The nuclear genome assembly has a scaffold N50 of 34.33 Mb, a total assembly span of 775.53 Mb and 99.63% of the sequence assembled into 24 superscaffolds, consistent with its known karyotype. Quality metrics revealed a consensus accuracy (QV) of 42.92 and gene completeness > 98%. The genome annotation resulted in 26,690 protein-coding genes and 12,737 non-coding transcripts. The coding regions encoded 39,613 unique protein products, 93% of them with assigned function. Overall, the publication of the X. novacula's reference genome will broaden the scope and impact of genomic research conducted on this iconic and colourful species. (© The Author(s) 2023. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.)
Databáze:	MEDLINE
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