Early gonadogenesis in Columba livia (birds: Columbiformes): Migration, colonization, and differentiation of germ cells.

Autor: Olea GB; Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional del Nordeste, Resistencia, Argentina.; Cátedra de Histología y Embriología. Departamento de Ciencias Básicas, Universidad Nacional del Chaco Austral, Sáenz Peña, Argentina.; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina., Aguirre MV; Laboratorio de Investigaciones Bioquímicas de la Facultad de Medicina (LIBIM) Facultad de Medicina, Universidad Nacional del Nordeste, Instituto de Química Básica y Aplicada del NEA, (IQUIBA NEA-UNNE- CONICET), Resistencia, Argentina., Lombardo DM; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina.; Universidad de Buenos Aires, Facultad de Cs Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal (INITRA). Cátedra de Histología y Embriología, Viamonte, Argentina.
Jazyk: angličtina
Zdroj: Development, growth & differentiation [Dev Growth Differ] 2024 Jan; Vol. 66 (1), pp. 56-65. Date of Electronic Publication: 2023 Oct 17.
DOI: 10.1111/dgd.12895
Abstrakt: In birds, primordial germ cells (PGCs) use the bloodstream to travel to a specific region, where the cells undergo extravasation followed by intrastromal migration to the gonadal crest for further colonization. Currently, DDX4, SSEA1, and Oct4 are used to identify germ cells. Other germline cell-associated molecules are N-cadherin, GnRHR, and 3β hydroxysteroid dehydrogenase (3βHSD), which have been used in mice and birds during gonadal development; however, its role in early gonadogenesis in birds is poorly described. This study aimed to evaluate the differential immunodetection of N-cadherin binding molecule, Oct4 pluripotency protein, GnRHR receptor, and 3βHSD enzyme in Columba livia embryos during migration colonization of PGCs in the gonadal crest and early gonadogenesis. These markers were revealed by immunohistochemistry in histological preparations of C. livia corresponding to stages (S)15 to S40. Immunodetection of N-cadherin, Oct4, GnRHR, and 3βHSD in the germ line of C. livia allowed the identification of PGCs in the yolk sac membrane at the level of the splanchnic mesoderm during migration to the genital crest and its colonization. In the same way, it was possible to characterize and localize PGCs during early gonadogenesis. This study in C. livia demonstrates that Oct4, N-cadherin, GNRHR, and 3βHSD are immunodetected in PGCs and could be used as potential germline cell markers during cell migration out of blood vessels, colonization in the genital crest, and early gonadogenesis. Furthermore, this study could be used as a novel general model to understand the early gonadogenesis in altricial species.
(© 2023 Japanese Society of Developmental Biologists.)
Databáze: MEDLINE
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