Quantification and comparison of salivary neutrophils in periodontal health and disease.

Autor:	Chakravarthy Reddy YS; Department of Periodontics, Lenora Institute of Dental Sciences, Rajahmundry, Andhra Pradesh, India., Koppolu P; Dental School, The University of Western Australia, Perth, WA, Australia., Alotaibi DH; Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, KSA., Alsinaidi AA; Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh, KSA., Mukhtar AH; Periodontics Division, Department of Preventive Dentistry, College of Dentistry, Riyadh Elm University, Riyadh, KSA., Kakti AA; Pedodontics Division, Department of Preventive dentistry, College of Dentistry, Riyadh Elm University, Riyadh, KSA., Abdelrahim RK; Department of Preventive Dental Sciences, College of Dentistry, Dar Al Uloom University, Riyadh, Saudi Arabia., Lingam AS; Department of Surgical and Diagnostic Sciences, Dar Al Uloom University, Riyadh, Saudi Arabia.
Jazyk:	angličtina
Zdroj:	Nigerian journal of clinical practice [Niger J Clin Pract] 2023 Sep; Vol. 26 (9), pp. 1283-1289.
DOI:	10.4103/njcp.njcp_3_23
Abstrakt:	Background: Neutrophils continuously migrate into the oral cavity from various sources like gingival crevicular fluid and saliva both in health and in inflammation. The migration of the neutrophils into the various tissues and into the oral cavity occurs when the host microbial interplay tips the balance favoring the initiation of the inflammatory and immune reactions which depending on the amount of the microbial load results in the development of acute and chronic infections in the susceptible host. Aim: The present study was designed to quantify and compare the oral salivary neutrophil levels in patients with gingivitis and chronic and aggressive periodontitis as well as in healthy controls, before and after scaling and root planing (SRP) and to compare the difference within the selected study groups. Materials and Methods: Forty subjects were classified into four groups, that is, healthy controls, gingivitis, and chronic and aggressive periodontitis. Oral rinse samples were collected using Hank's balanced salt solution from each patient before and after phase I periodontal therapy. Cells in the rinse samples were stained with Acridine orange, and neutrophil counts were carried out using a fluorescence microscope and a hemocytometer. Results: Baseline oral salivary neutrophil levels were maximum in the chronic periodontitis group followed by the aggressive group and then the gingivitis group. Oral salivary neutrophil levels also positively correlated to probing pocket depth, plaque index, calculus index, and gingival index in all four study groups. Maximum reduction in the oral salivary neutrophil levels after phase I periodontal therapy was seen in the gingivitis group. Conclusion: From our study, we conclude that the oral salivary neutrophil levels decreased significantly after SRP. Estimation of changes in the oral salivary neutrophil levels has the potential to aid in monitoring treatment outcomes. Thus, it suggests that it could be used as a simple, noninvasive laboratory technique to monitor the periodontal status and disease progression. Competing Interests: None
Databáze:	MEDLINE
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