Structural characterization, and in vitro hypoglycemic activity of a polysaccharide from the mushroom Cantharellus yunnanensis.

Autor: Tian R; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China., Zhang YZ; Gongyi Public Traditional Chinese Medicine Hospital, Henan Province, Zhengzhou 451200, China., Cheng X; Dietary Fiber Isolation and Structural Characterization Laboratory, Guangxi Vocational College of Technology and Business, Nanning 530003, China., Xu B; Food Science and Technology Program, Department of Life Sciences, BNU-HKBU United International College, Zhuhai, Guangdong 519087, China., Wu H; Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Liang ZQ; College of Science, Hainan University, Haikou 570228, China., Rahman M; General Hospital of the Third Division of Xinjiang Production and Construction Corps, Kashgar Hospital District, Kashgar 844000, China., Wang Y; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China. Electronic address: wangyong1982_2004@163.com., Zeng NK; Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China. Electronic address: niankaiz@163.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Dec 31; Vol. 253 (Pt 5), pp. 127200. Date of Electronic Publication: 2023 Oct 02.
DOI: 10.1016/j.ijbiomac.2023.127200
Abstrakt: A polysaccharide CY-2 from C. yunnanensis was obtained through a process of consecutive water extraction, alcohol precipitation, and DEAE-52 fast-flow chromatography. CY-2, with an average molecular weight of 2.69 × 10 4  Da mainly consisted of glucose and mannose with a molar ratio of 33.5: 56.9. Infrared spectrum (IR), methylation analysis, and nuclear magnetic resonance (NMR) results revealed that CY-2 may have a backbone consisting of →6)-α-D-Manp-(1 → 3)-β-D-Glcp-(1→, and branch chain β-D-Glcp-(1→. Meanwhile, CY-2 had a higher inhibition rate on α-glucosidase activity compared with other fractions (CY-0, CY-1, and CY-4) and was a mixed competitive inhibitor. In addition, CY-2 at the concentration of 10 μg/mL presented a superior power to improve glucose consumption and metabolism in HepG2 cells compared with metformin. Overall, these findings highlight the potential value of CY-2 as a hypoglycemic agent.
Competing Interests: Declaration of competing interest The authors confirm that there are no known conflicts of interest associated with this publication.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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