Appraisals on the chemical characterization and biological potentials of Ranunculus constantinopolitanus extracts using chromatographic, computational, and molecular network approaches.

Autor: Lazarova I; Department of Chemistry, Faculty of Pharmacy, Medical University-Sofia, 2, Dunav Str., 1000 Sofia, Bulgaria., Zengin G; Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr., Piatti D; CHemistry Interdisciplinary Project (CHip), School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy., Uba AI; Department of Molecular Biology and Genetics, Istanbul AREL University, Istanbul 34537, Turkey., Sagratini G; CHemistry Interdisciplinary Project (CHip), School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy., Caprioli G; CHemistry Interdisciplinary Project (CHip), School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy., Emre G; Department of Pharmaceutical Botany, Pharmacy Faculty, Marmara University, Istanbul, Turkey., Ponniya SKM; Laboratory of Natural Products and Medicinal Chemistry (LNPMC), Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, 600077, India., Rengasamy KR; Laboratory of Natural Products and Medicinal Chemistry (LNPMC), Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, 600077, India., Paradis NJ; College of Science and Mathematics, Rowan University, Glassboro, NJ 08028, USA., Koyuncu I; Department of Medical Biochemistry, Faculty of Medicine, Harran University, Sanliurfa 63290, Turkey., Şeker F; Department of Biology, Science Arts Faculty, Harran University, Sanliurfa, Turkey., Wu C; College of Science and Mathematics, Rowan University, Glassboro, NJ 08028, USA., Nilofar; Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, Konya, Turkey; Department of Pharmacy, Botanic Garden 'Giardino dei Semplici', 'Gabriele d'Annunzio' University, 66100 Chieti, Italy., Flores GA; Department of Pharmacy, Botanic Garden 'Giardino dei Semplici', 'Gabriele d'Annunzio' University, 66100 Chieti, Italy., Cusumano G; Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia (PG), Italy., Angelini P; Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia (PG), Italy., Venanzoni R; Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia (PG), Italy.
Jazyk: angličtina
Zdroj: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2023 Nov; Vol. 181, pp. 114064. Date of Electronic Publication: 2023 Oct 02.
DOI: 10.1016/j.fct.2023.114064
Abstrakt: In this context, phytochemicals were extracted from Ranunculus constantinopolitanus using ethyl acetate (EA), ethanol, ethanol/water (70%), and water solvent. The analysis encompassed quantification of total phenolic and flavonoid content using spectrophotometric assays, chemical profiling via high performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) for the extracts, and assessment of antioxidant activity via 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), Cupric reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), metal chelating (MCA), and phosphomolybdenum (PBD) assays. Moreover, antimicrobial activity was assessed against four different bacterial strains, as well as various yeasts. Enzyme inhibitory activities were evaluated against five types of enzymes. Additionally, the extracts were examined for their anticancer and protective effects on several cancer cell lines and the human normal cell line. All of the extracts exhibited significant levels of ferulic acid, kaempferol, and caffeic acid. All tested extracts demonstrated antimicrobial activity, with Escherichia coli and Pseudomonas aeruginosa being most sensitive to EA and ethanol extracts. Molecular docking studies revealed that kaempferol-3-O-glucoside strong interactions with AChE, BChE and tyrosinase. In addition, network pharmacology showed an association between gastric cancer and kaempferol-3-O-glucoside. Based on the results, R. constantinopolitanus can be a potential reservoir of bioactive compounds for future bioproduct innovation and pharmaceutical industries.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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