Plasma-generated nitric oxide water: A promising strategy to combat bacterial dormancy (VBNC state) in environmental contaminant Micrococcus luteus.

Autor: Borkar SB; Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea., Negi M; Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea., Jaiswal A; Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea., Raj Acharya T; Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea., Kaushik N; Department of Biotechnology, College of Engineering, The University of Suwon, Hwaseong 18323, South Korea. Electronic address: neha.bioplasma@gmail.com., Choi EH; Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea. Electronic address: ehchoi@kw.ac.kr., Kaushik NK; Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea. Electronic address: kaushik.nagendra@kw.ac.kr.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2024 Jan 05; Vol. 461, pp. 132634. Date of Electronic Publication: 2023 Sep 27.
DOI: 10.1016/j.jhazmat.2023.132634
Abstrakt: The viable but non-culturable (VBNC) is an inactive state, and certain bacteria can enter under adverse conditions. The VBNC state challenges the environment, food safety, and public health since VBNCs may resuscitate and pose a risk to human health. The aim of this study was to investigate the effect of plasma-generated nitric oxide water (PG-NOW) on airborne contaminant Micrococcus luteus (M. luteus) and examine its potential to induce the VBNC state. The essential conditions for bacteria to enter VBNC state are low metabolic activity and rare or no culturable counts. The results indicated that PG-NOW effectively eliminates M. luteus, and the remaining bacteria are in culturable condition. Moreover, the conventional cultured-based method combined with a propidium iodide monoazide quantitative PCR (PMAxx TM -qPCR) showed no significant VBNC induction and moderate culturable counts. Results from the qPCR revealed that gene levels in PG-NOW treated bacteria related to resuscitation-promoting factors, amino acid biosynthesis, and fatty acid metabolism were notably upregulated. PG-NOW inactivated M. luteus showed negligible VBNC formation and alleviated infection ability in lung cells. This study provides new insights into the potential use of PG-NOW reactive species for the prevention and control of the VBNC state of M. luteus.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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