| Autor: |
Tang Z; School of Pharmacy, Wannan Medical College, Wuhu, China.; Department of Pharmacy, Huzhou Central Hospital, Huzhou, China., Zhang M; School of Pharmacy, Wannan Medical College, Wuhu, China.; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macromolecules, Wuhu, China., Gao L; School of Pharmacy, Wannan Medical College, Wuhu, China., Bao Y; School of Pharmacy, Wannan Medical College, Wuhu, China., Li P; School of Pharmacy, Wannan Medical College, Wuhu, China.; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macromolecules, Wuhu, China., Wang M; Wuhu Medical and Health School, Wuhu, China., Shao T; School of Pharmacy, Wannan Medical College, Wuhu, China.; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macromolecules, Wuhu, China., Wang G; School of Pharmacy, Wannan Medical College, Wuhu, China.; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macromolecules, Wuhu, China.; Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui, Anhui Innovative Center for Drug Basic Research of Metabolic Diseases, Wuhu, China., Liu C; School of Pharmacy, Wannan Medical College, Wuhu, China.; Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macromolecules, Wuhu, China.; Anhui Provincial Engineering Laboratory for Screening and Re-evaluation of Active Compounds of Herbal Medicines in Southern Anhui, Anhui Innovative Center for Drug Basic Research of Metabolic Diseases, Wuhu, China. |
| Abstrakt: |
Stevia rebaudiana boasts a wide range of medical and food applications and contains polysaccharides that exert beneficial effects against oxidative stress. In this study, we optimised the extraction of a polysaccharide (SRRP) from S. rebaudiana roots by employing a Box-Behnken design and response surface methodology. The optimal extraction conditions were as follows: 93.57 min, 71.67 °C, and a water-to-raw material ratio of 21.40 mL/g. Under these conditions, 14.00 ± 0.35% of crude polysaccharide was obtained. Treatment of RAW264.7 cells with SRRP prior to the addition of H 2 O 2 , a major contributor to oxidative damage, significantly increased cell viability. In addition, SRRP increased the levels of superoxide dismutase, catalase, and glutathione and reduced the levels of malondialdehyde in RAW264.7 cells. Therefore, SRRP can provide effective protection against H 2 O 2 -induced oxidative damage. These findings indicate the potential of SRRP as a natural antioxidant in the food and pharmaceutical industries. |
