Partnering essential oils with antibiotics: proven therapies against bovine Staphylococcus aureus mastitis.

Autor:	Abd El-Hamid MI; Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., El-Tarabili RM; Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt., Bahnass MM; Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.; Department of Clinical Laboratory Sciences, Applied Medical Sciences College, Najran University, Najran, Saudi Arabia., Alshahrani MA; Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, Najran, Saudi Arabia., Saif A; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia., Alwutayd KM; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia., Safhi FA; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia., Mansour AT; Animal and Fish Production Department, College of Agricultural and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia.; Fish and Animal Production Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt., Alblwi NAN; Al Hadithah General Hospital, Al-Qurayyat, Saudi Arabia., Ghoneim MM; Department of Pharmacy Practice, College of Pharmacy, Al Maarefa University, Riyadh, Saudi Arabia., Elmaaty AA; Medicinal Chemistry Department, Faculty of Pharmacy, Port Said University, Port Said, Egypt., Al-Harthi HF; Biology Department, Turabah University College, Taif University, Taif, Saudi Arabia., Bendary MM; Department of Microbiology and Immunology, Faculty of Pharmacy, Port Said University, Port Said, Egypt.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2023 Sep 15; Vol. 13, pp. 1265027. Date of Electronic Publication: 2023 Sep 15 (Print Publication: 2023).
DOI:	10.3389/fcimb.2023.1265027
Abstrakt:	Introduction: There is an urgent need to develop therapeutic options for biofilm-producing Staphylococcus aureus (S. aureus). Therefore, the renewed interest in essential oils (EOs), especially carvacrol, linalool and eugenol, has attracted the attention of our research group. Methods: Multidrug resistance and multivirulence profiles in addition to biofilm production of S. aureus strains isolated from cows with mastitis were evaluated using both phenotypic and genotypic methods. The antimicrobial and antibiofilm activities of EOs were tested using both in vitro and molecular docking studies. Moreover, the interactions between commonly used antibiotics and the tested EOs were detected using the checkerboard method. Results: We found that all our isolates (n= 37) were biofilm methicillin resistant S. aureus (MRSA) producers and 40.5% were vancomycin resistant S. aureus (VRSA). Unfortunately, 73 and 43.2% of the recovered MRSA isolates showed multidrug resistant (MDR) and multivirulence patterns, respectively. The antimicrobial activities of the tested EOs matched with the phenotypic evaluation of the antibiofilm activities and molecular docking studies. Linalool showed the highest antimicrobial and antibiofilm activities, followed by carvacrol and eugenol EOs. Fortunately, synergistic interactions between the investigated EOs and methicillin or vancomycin were detected with fractional inhibitory concentration index (FICI) values ≤ 0.5. Moreover, the antimicrobial resistance patterns of 13 isolates changed to sensitive phenotypes after treatment with any of the investigated EOs. Treatment failure of bovine mastitis with resistant S. aureus can be avoided by combining the investigated EOs with available antimicrobial drugs. Conclusion: We hope that our findings can be translated into a formulation of new pharmaceutical dosage forms against biofilm-producing S. aureus pathogens. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Abd El-Hamid, El-Tarabili, Bahnass, Alshahrani, Saif, Alwutayd, Safhi, Mansour, Alblwi, Ghoneim, Elmaaty, Al-harthi and Bendary.)
Databáze:	MEDLINE
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