Gut content metabarcoding of specialized feeders is not a replacement for environmental DNA assays of seawater in reef environments.
| Autor: | DiBattista JD; School of Molecular and Life Sciences, Curtin University, Perth, WA, Australia.; Australian Museum Research Institute, Australian Museum, Sydney, NSW, Australia., Liu SYV; Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan., De Brauwer M; CSIRO Oceans and Atmosphere, Hobart, TAS, Australia., Wilkinson SP; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand., West K; CSIRO Australian National Fish Collection, CSIRO, Hobart, TAS, Australia., Koziol A; School of Molecular and Life Sciences, Curtin University, Perth, WA, Australia.; Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark., Bunce M; Institute of Environmental Science and Research, Kenepuru, Porirua, New Zealand. |
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| Jazyk: | angličtina |
| Zdroj: | PeerJ [PeerJ] 2023 Sep 27; Vol. 11, pp. e16075. Date of Electronic Publication: 2023 Sep 27 (Print Publication: 2023). |
| DOI: | 10.7717/peerj.16075 |
| Abstrakt: | In tropical marine ecosystems, the coral-based diet of benthic-feeding reef fishes provides a window into the composition and health of coral reefs. In this study, for the first time, we compare multi-assay metabarcoding sequences of environmental DNA (eDNA) isolated from seawater and partially digested gut items from an obligate corallivore butterflyfish ( Chaetodon lunulatus ) resident to coral reef sites in the South China Sea. We specifically tested the proportional and statistical overlap of the different approaches (seawater vs gut content metabarcoding) in characterizing eukaryotic community composition on coral reefs. Based on 18S and ITS2 sequence data, which differed in their taxonomic sensitivity, we found that gut content detections were only partially representative of the eukaryotic communities detected in the seawater based on low levels of taxonomic overlap (3 to 21%) and significant differences between the sampling approaches. Overall, our results indicate that dietary metabarcoding of specialized feeders can be complimentary to, but is no replacement for, more comprehensive environmental DNA assays of reef environments that might include the processing of different substrates (seawater, sediment, plankton) or traditional observational surveys. These molecular assays, in tandem, might be best suited to highly productive but cryptic oceanic environments (kelp forests, seagrass meadows) that contain an abundance of organisms that are often small, epiphytic, symbiotic, or cryptic. Competing Interests: The authors declare that they have no competing interests. (© 2023 DiBattista et al.) |
| Databáze: | MEDLINE |
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