A metagenomic comparison of clearwater, probiotic, and Rapid BFT TM on Pacific whiteleg shrimp, Litopenaeus vannamei cultures.

Autor:	Waiho K; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.; Centre for Chemical Biology, Universiti Sains Malaysia, Minden, Penang, Malaysia.; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, Guangdong, China., Abd Razak MS; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.; Zaiyadal Aquaculture Sdn. Bhd., Shah Alam, Selangor, Malaysia., Abdul Rahman MZ; Zaiyadal Aquaculture Sdn. Bhd., Shah Alam, Selangor, Malaysia., Zaid Z; Zaiyadal Aquaculture Sdn. Bhd., Shah Alam, Selangor, Malaysia., Ikhwanuddin M; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, Guangdong, China.; Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, Indonesia., Fazhan H; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.; Centre for Chemical Biology, Universiti Sains Malaysia, Minden, Penang, Malaysia.; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, Guangdong, China., Shu-Chien AC; Centre for Chemical Biology, Universiti Sains Malaysia, Minden, Penang, Malaysia.; School of Biological Sciences, Universiti Sains Malaysia, Minden, Penang, Malaysia., Lau NS; Centre for Chemical Biology, Universiti Sains Malaysia, Minden, Penang, Malaysia., Azmie G; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia., Ishak AN; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia., Syahnon M; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.; Centre of Research and Field Service (CRaFS), Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia., Kasan NA; Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Malaysia.; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, Guangdong, China.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Sep 28; Vol. 11, pp. e15758. Date of Electronic Publication: 2023 Sep 28 (Print Publication: 2023).
DOI:	10.7717/peerj.15758
Abstrakt:	Biofloc technology improves water quality and promote the growth of beneficial bacteria community in shrimp culture. However, little is known about the bacteria community structure in both water and gut of cultured organisms. To address this, the current study characterised the metagenomes derived from water and shrimp intestine samples of novel Rapid BFT TM with probiotic and clearwater treatments using 16S V4 region and full length 16S sequencing. Bacteria diversity of water and intestine samples of Rapid BFT TM and probiotic treatments were similar. Based on the 16S V4 region, water samples of >20 μm biofloc had the highest abundance of amplicon sequence variant (ASV). However, based on full length 16S, no clear distinction in microbial diversity was observed between water samples and intestine samples. Proteobacteria was the most abundant taxon in all samples based on both 16S V4 and full length 16S sequences. Vibrio was among the highest genus based on 16S V4 region but only full length 16S was able to discern up to species level, with three Vibrio s identified- V. harveyi , V. parahaemolyticus and V. vulnificus . Vibrio harveyi being the most abundant species in all treatments. Among water samples, biofloc water samples had the lowest abundance of all three Vibrio s, with V. vulnificus was present only in bioflocs of <20 μm. Predicted functional profiles of treatments support the beneficial impacts of probiotic and biofloc inclusion into shrimp culture system. This study highlights the potential displacement of opportunistic pathogens by the usage of biofloc technology (Rapid BFT TM ) in shrimp culture. Competing Interests: Khor Waiho is an Academic Editor for PeerJ. Muhammad Syafiq Abd Razak, Mohd Zaidy Abdul Rahman, and Zainah Zaid are employees of Zaiyadal Aquaculture Sdn. Bhd. (© 2023 Waiho et al.)
Databáze:	MEDLINE
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