Comparative Assessment of SSR and RAPD markers for genetic diversity in some Mango cultivars.

Autor:	A A Hussein M; Department of Botany (Genetic Branch), Faculty of Agriculture, Suez Canal University, Ismailia, Egypt., Eid M; Department of Botany (Genetic Branch), Faculty of Agriculture, Suez Canal University, Ismailia, Egypt., Rahimi M; Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran., Filimban FZ; Division of Botany, Department of Biology, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia., Abd El-Moneim D; Faculty of Environmental Agricultural Sciences, Plant Production Department - Genetic Branch, Arish University, El Arish, Egypt.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Sep 28; Vol. 11, pp. e15722. Date of Electronic Publication: 2023 Sep 28 (Print Publication: 2023).
DOI:	10.7717/peerj.15722
Abstrakt:	Genetic improvement mainly depends on the level of genetic variability present in the population, and the degree of genetic diversity in a population largely determines the rate of genetic advancement. For analyzing genetic diversity and determining cultivar identities, a molecular marker is a useful tool. Using 30 SSR (simple sequence repeat) and 30 RAPD (randomly amplified polymorphic DNA) markers, this study evaluated the genetic divergence of 17 mango cultivars. The effectiveness of the two marker systems was evaluated using their genetic diversity characteristics. Additionally, the effects of SM (simple matching) and Dice similarity coefficients and their effects on mango clustering were evaluated. The findings showed that SSR markers generated 192 alleles, all of which were polymorphic (100%). With RAPD markers, 434 bands were obtained, 361 of which were polymorphic (83%). The average polymorphic information content (PIC) for RAPD and SSR was 0.378 and 0.735, respectively. Using SSR markers resulted in much higher values for other genetic diversity parameters compared to RAPD markers. Furthermore, grouping the genotypes according to the two similarity coefficients without detailed consideration of these coefficients could not influence the study results. The RAPD markers OPA_01, OPM_12 followed by OPO_12 and SSR markers MIAC_4, MIAC_5 followed by mMiCIR_21 were the most informative in terms of describing genetic variability among the cultivars under study; they can be used in further investigations such as genetic mapping or marker-assisted selection. Overall, 'Zebda' cultivar was the most diverse of the studied cultivars. Competing Interests: Diaa Abd El-Moneim is an Academic Editor for PeerJ. (©2023 A. A. Hussein et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu