Covalent functionalisation controlled by molecular design for the aptameric recognition of serotonin in graphene-based field-effect transistors.

Autor: Wetzl C; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, 20014, Donostia-San Sebastián, Spain. mprato@cicbiomagune.es.; University of the Basque Country, UPV-EHU, 20018 San Sebastián, Spain., Brosel-Oliu S; Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Spain. elisabet.prats@csic.es., Carini M; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, 20014, Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Di Silvio D; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, 20014, Donostia-San Sebastián, Spain. mprato@cicbiomagune.es., Illa X; Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Spain. elisabet.prats@csic.es.; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain., Villa R; Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Spain. elisabet.prats@csic.es.; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain., Guimera A; Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Spain. elisabet.prats@csic.es.; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain., Prats-Alfonso E; Instituto de Microelectrónica de Barcelona, IMB-CNM (CSIC), Campus UAB, Bellaterra, Spain. elisabet.prats@csic.es.; Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina, Instituto de Salud Carlos III, Spain., Prato M; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, 20014, Donostia-San Sebastián, Spain. mprato@cicbiomagune.es.; Ikerbasque, Basque Foundation for Science, Bilbao, Spain.; Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, Trieste, Italy., Criado A; Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Paseo de Miramón 194, 20014, Donostia-San Sebastián, Spain. mprato@cicbiomagune.es.; Universidade da Coruña, CICA - Centro Interdisciplinar de Química e Bioloxía, Rúa as Carballeiras, 15071 A Coruña, Spain. a.criado@udc.es.
Jazyk: angličtina
Zdroj: Nanoscale [Nanoscale] 2023 Oct 26; Vol. 15 (41), pp. 16650-16657. Date of Electronic Publication: 2023 Oct 26.
DOI: 10.1039/d3nr04153k
Abstrakt: In the last decade, solution-gated graphene field effect transistors (GFETs) showed their versatility in the development of a miniaturized multiplexed platform for electrophysiological recordings and sensing. Due to their working mechanism, the surface functionalisation and immobilisation of receptors are pivotal to ensure the proper functioning of devices. Herein, we present a controlled covalent functionalisation strategy based on molecular design and electrochemical triggering, which provide a monolayer-like functionalisation of micro-GFET arrays retaining the electronic properties of graphenes. The functionalisation layer as a receptor was then employed as the linker for serotonin aptamer conjugation. The micro-GFET arrays display sensitivity toward the target analyte in the micromolar range in a physiological buffer (PBS 10 mM). The sensor allows the in-flow real-time monitoring of serotonin transient concentrations with fast and reversible responses.
Databáze: MEDLINE
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