Association of 2-oxoacid dehydrogenase complexes with respirasomes in mitochondria.

Autor: Plokhikh KS; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia.; Shubnikov Institute of Crystallography, Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russia., Nesterov SV; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia.; Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Chesnokov YM; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia.; Shubnikov Institute of Crystallography, Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russia., Rogov AG; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia., Kamyshinsky RA; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia.; Shubnikov Institute of Crystallography, Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russia., Vasiliev AL; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia.; Shubnikov Institute of Crystallography, Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Moscow, Russia.; Moscow Institute of Physics and Technology, Dolgoprudny, Russia., Yaguzhinsky LS; Moscow Institute of Physics and Technology, Dolgoprudny, Russia.; Belozersky Research Institute for Physico-Chemical Biology, Lomonosov Moscow State University, Russia., Vasilov RG; Kurchatov Complex of NBICS-Technologies, National Research Center Kurchatov Institute, Moscow, Russia.
Jazyk: angličtina
Zdroj: The FEBS journal [FEBS J] 2024 Jan; Vol. 291 (1), pp. 132-141. Date of Electronic Publication: 2023 Oct 15.
DOI: 10.1111/febs.16965
Abstrakt: In the present study, cryo-electron tomography was used to investigate the localization of 2-oxoacid dehydrogenase complexes (OADCs) in cardiac mitochondria and mitochondrial inner membrane samples. Two classes of ordered OADC inner cores with different symmetries were distinguished and their quaternary structures modeled. One class corresponds to pyruvate dehydrogenase complexes and the other to dehydrogenase complexes of α-ketoglutarate and branched-chain α-ketoacids. OADCs were shown to be localized in close proximity to membrane-embedded respirasomes, as observed both in densely packed lamellar cristae of cardiac mitochondria and in ruptured mitochondrial samples where the dense packing is absent. This suggests the specificity of the OADC-respirasome interaction, which allows localized NADH/NAD + exchange between OADCs and complex I of the respiratory chain. The importance of this local coupling is based on OADCs being the link between respiration, glycolysis and amino acid metabolism. The coupling of these basic metabolic processes can vary in different tissues and conditions and may be involved in the development of various pathologies. The present study shows that this important and previously missing parameter of mitochondrial complex coupling can be successfully assessed using cryo-electron tomography.
(© 2023 Federation of European Biochemical Societies.)
Databáze: MEDLINE
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