Anti-HDV reflex testing in HBsAg-positive subjects: An efficacious strategy to identify HDV infection.

Autor: Cossiga V; Department of Clinical Medicine and Surgery, Diseases of the Liver and Biliary System Unit, University of Naples 'Federico II', Naples, Italy., Brusa S; Department of Translational Medical Science, University of Naples 'Federico II', Naples, Italy., Montalti R; Department of Public Health, Division of Hepato-Bilio-Pancreatic, Minimally Invasive and Robotic Surgery, University of Naples 'Federico II', Naples, Italy., De Conte A; Department of Clinical Medicine and Surgery, Diseases of the Liver and Biliary System Unit, University of Naples 'Federico II', Naples, Italy., Jannuzzi G; Department of Translational Medical Science, University of Naples 'Federico II', Naples, Italy., Ranieri L; Department of Clinical Medicine and Surgery, Diseases of the Liver and Biliary System Unit, University of Naples 'Federico II', Naples, Italy., Sorrentino R; Department of Translational Medical Science, University of Naples 'Federico II', Naples, Italy., Vallefuoco L; Department of Translational Medical Science, University of Naples 'Federico II', Naples, Italy., Pignata L; Department of Clinical Medicine and Surgery, Diseases of the Liver and Biliary System Unit, University of Naples 'Federico II', Naples, Italy., Guarino M; Department of Clinical Medicine and Surgery, Diseases of the Liver and Biliary System Unit, University of Naples 'Federico II', Naples, Italy., Portella G; Department of Translational Medical Science, University of Naples 'Federico II', Naples, Italy., Morisco F; Department of Clinical Medicine and Surgery, Diseases of the Liver and Biliary System Unit, University of Naples 'Federico II', Naples, Italy.
Jazyk: angličtina
Zdroj: Liver international : official journal of the International Association for the Study of the Liver [Liver Int] 2024 Jan; Vol. 44 (1), pp. 148-154. Date of Electronic Publication: 2023 Oct 03.
DOI: 10.1111/liv.15746
Abstrakt: Background and Aims: The prevalence of HDV infection in HBsAg carriers is about 9.9% in Italy. However, the real prevalence is underestimated because the anti-HDV test is not performed routinely in all HBsAg carriers. The aim of this study was to compare the prevalence and the absolute number of HDV infection identified in HBsAg-positive subjects tested at University Hospital Federico II before and after the introduction of anti-HDV reflex testing.
Methods: From January to December 2022, reflex test for the detection of total HDV antibodies was performed in all HBsAg-positive subjects tested at University Hospital Federico II. The control group consisted of all the HBsAg-positive subjects tested at the same laboratory in 2019, before the implementation of anti-HDV reflex testing. Sera were evaluated with ADVIA Centaur HBsAgII Qualitative, Liaison Murex HBsAg Quantitative and Liaison Murex Total Anti-HDV Qualitative.
Results: Before reflex testing, anti-HDV had been tested in 16.4% (84/512) of HBsAg-positive subjects, while after its implementation, 100% (484/484) of HBsAg-positive patients was tested for anti-HDV. The anti-HDV positive prevalence was lower than before the introduction of reflex test (10.7% vs. 16.6%) but the absolute number of anti-HDV positive patients increased (14 vs. 52 subjects). HDV-RNA was detectable in 26 (53%) of 49 tested subjects.
Conclusions: Our data showed that the implementation of anti-HDV reflex testing increased the diagnoses of HDV infection. In this setting, due to the approval of specific anti-HDV drugs, a reflex test for anti-HDV should be implemented to early identify patients with HBV/HDV infection.
(© 2023 The Authors. Liver International published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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