Whole genome sequencing of increased number of azithromycin-resistant Shigella flexneri 1b isolates in Ontario.

Autor: Neemuchwala A; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Zittermann S; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Johnson K; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Middleton D; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Stapleton PJ; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Ravirajan V; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Cronin K; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada., Allen VG; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.; Sinai Health, Mount Sinai Hospital, Toronto, ON, Canada., Patel SN; Ontario Agency for Health Protection and Promotion (Public Health Ontario), Toronto, ON, Canada. Samir.Patel@oahpp.ca.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada. Samir.Patel@oahpp.ca.; Microbiology and Laboratory Science, Public Health Ontario, 661 University Ave, Suite 1701, Toronto, ON, M5G 1M1, Canada. Samir.Patel@oahpp.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Oct 03; Vol. 13 (1), pp. 16582. Date of Electronic Publication: 2023 Oct 03.
DOI: 10.1038/s41598-023-36733-w
Abstrakt: Azithromycin (AZM) resistance among Shigella is a major public health concern. Here, we investigated the epidemiology of Shigella flexneri serotype 1b recovered during 2016-2018 in Ontario, to describe the prevalence and spread of AZM resistance. We found that 72.3% (47/65) of cases were AZM-resistant (AZM R ), of which 95.7% (45/47) were among males (P < 0.001). Whole-genome based phylogenetic analysis showed three major clusters, and 56.9% of isolates grouped within a single closely-related cluster (0-10 ∆SNP). A single AZM R clonal population was persistent over 3 years and involved 67.9% (36/53) of all male cases, and none reported international travel. In 2018, a different AZM R cluster appeared among adult males not reporting travel. A proportion of isolates (10.7%) with reduced susceptibility to ciprofloxacin (CIP) due to S83L mutation in gyrA were AZM susceptible, and 71.4% reported international travel. Resistance to AZM was due to the acquisition of mph gene-bearing incFII plasmids having > 95% nucleotide similarity to pKSR100. Plasmid-borne resistance limiting treatment options to AZM, ceftriaxone (CRO) and CIP was noted in a single isolate. We characterized AZM R isolates circulating locally among males and found that genomic analysis can support targeted prevention and mitigation strategies against antimicrobial-resistance.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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