Discovery of a new highly pathogenic toxin involved in insect sepsis.

Autor:	Zhang Y; MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University , Beijing, China., Li H; MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University , Beijing, China., Wang F; Institute of Plant Protection, Ningxia Academy of Agricultural and Forestry Sciences , Yinchuan, China., Liu C; Institute of Plant Protection, Ningxia Academy of Agricultural and Forestry Sciences , Yinchuan, China., Reddy GVP; Department of Entomology, Lousiana State University , Baton Rouge, Los Angeles, USA., Li H; MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University , Beijing, China.; Sanya Institute of China Agricultural University, China Agricultural University , Sanya, China., Li Z; MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University , Beijing, China.; Sanya Institute of China Agricultural University, China Agricultural University , Sanya, China., Sun Y; Institute of Zoology, Chinese Academy of Sciences , Beijing, China., Zhao Z; MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University , Beijing, China.; Sanya Institute of China Agricultural University, China Agricultural University , Sanya, China.
Jazyk:	angličtina
Zdroj:	Microbiology spectrum [Microbiol Spectr] 2023 Dec 12; Vol. 11 (6), pp. e0142223. Date of Electronic Publication: 2023 Oct 03.
DOI:	10.1128/spectrum.01422-23
Abstrakt:	Importance: As a current biocontrol resource, entomopathogenic nematodes and their symbiotic bacterium can produce many toxin factors to trigger insect sepsis, having the potential to promote sustainable pest management. In this study, we found Steinernema feltiae and Xenorhabdus bovienii were highly virulent against the insects. After infective juvenile injection, Galleria mellonella quickly turned black and softened with increasing esterase activity. Simultaneously, X. bovienii attacked hemocytes and released toxic components, resulting in extensive hemolysis and sepsis. Then, we applied high-resolution mass spectrometry-based metabolomics and found multiple substances were upregulated in the host hemolymph. We found extremely hazardous actinomycin D produced via 3-hydroxyanthranilic acid metabolites. Moreover, a combined transcriptomic analysis revealed that gene expression of proteins associated with actinomycin D was upregulated. Our research revealed actinomycin D might be responsible for the infestation activity of X. bovienii , indicating a new direction for exploring the sepsis mechanism and developing novel biotic pesticides. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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