Impact of leptin deficiency on male tibia and vertebral body 3D bone architecture independent of changes in body weight.

Autor: Williamson A; Biomolecular Science Research Centre, Department of Bioscience and Chemistry, Sheffield Hallam University, Sheffield, UK., da Silva A; Mississippi Center for Obesity Research, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA., do Carmo JM; Mississippi Center for Obesity Research, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA., Le Maitre CL; Biomolecular Science Research Centre, Department of Bioscience and Chemistry, Sheffield Hallam University, Sheffield, UK., Hall JE; Mississippi Center for Obesity Research, Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, USA., Aberdein N; Biomolecular Science Research Centre, Department of Bioscience and Chemistry, Sheffield Hallam University, Sheffield, UK.
Jazyk: angličtina
Zdroj: Physiological reports [Physiol Rep] 2023 Oct; Vol. 11 (19).
DOI: 10.14814/phy2.15832
Abstrakt: Leptin an adipokine with potent effects on energy balance and body weight plays an important role in defining bone architecture in growing mammals. However, major changes in body weight can also influence morphology of trabecular and cortical bone. Therefore, we examined the impact of leptin deficiency on tibia and vertebral body 3D bone architecture independent of changes in body weight. Furthermore, advances in computational 3D image analysis suggest that average morphological values may mask regional specific differences in trabecular bone thickness. The study utilized leptin-deficient Ob/Ob mice (n = 8) weight-paired to C57BL/6 (C57) control mice (n = 8) which were split into either lean or obese groups for 24 ± 2 weeks. Whole tibias and L3 vertebrae were fixed before high resolution microcomputed tomography (μCT) scanning was performed. Leptin deficiency independent of body weight reduced tibia cortical bone volume, trabecular bone volume/tissue volume, number, and mineral density. Mean tibia trabecular thickness showed no significant differences between all groups; however, significant changes in trabecular thickness were found when analyzed by region. This study demonstrates that leptin deficiency significantly impacts tibia and vertebral body trabecular and cortical bone 3D architecture independent of changes in body weight.
(© 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)
Databáze: MEDLINE
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