The genome of the colonial hydroid Hydractinia reveals their stem cells utilize a toolkit of evolutionarily shared genes with all animals.

Autor:	Schnitzler CE; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA.; Department of Biology, University of Florida, Gainesville, FL 32611, USA., Chang ES; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA., Waletich J; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA.; Department of Biology, University of Florida, Gainesville, FL 32611, USA., Quiroga-Artigas G; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA.; Department of Biology, University of Florida, Gainesville, FL 32611, USA.; Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier, Centre National de la Recherche Scientifique, 34293 Montpellier CEDEX 05, France., Wong WY; Department of Molecular Evolution and Development, Faculty of Life Science, University of Vienna, A-1090 Vienna, Austria., Nguyen AD; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Barreira SN; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Doonan L; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland., Gonzalez P; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Koren S; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Gahan JM; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland.; Department of Biochemistry, University of Oxford, Oxford, UK., Sanders SM; Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Pittsburgh Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA., Bradshaw B; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland., DuBuc TQ; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland.; Swarthmore College, Swarthmore, PA 19081, USA., Febrimarsa; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland., de Jong D; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA.; Department of Biology, University of Florida, Gainesville, FL 32611, USA., Nawrocki EP; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA., Larson A; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL 32080, USA., Klasfeld S; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Gornik SG; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland.; Centre for Organismal Studies, University of Heidelberg, Germany., Moreland RT; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Wolfsberg TG; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Phillippy AM; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA., Mullikin JC; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.; NIH Intramural Sequencing Center, Rockville, MD 20852, USA., Simakov O; Department of Molecular Evolution and Development, Faculty of Life Science, University of Vienna, A-1090 Vienna, Austria., Cartwright P; Department of Evolution and Ecology, University of Kansas, Lawrence, KS 66045, USA., Nicotra M; Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.; Pittsburgh Center for Evolutionary Biology and Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA., Frank U; Centre for Chromosome Biology, College of Science and Engineering, University of Galway, Galway, Ireland., Baxevanis AD; Division of Intramural Research, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Aug 27. Date of Electronic Publication: 2023 Aug 27.
DOI:	10.1101/2023.08.25.554815
Abstrakt:	Hydractinia is a colonial marine hydroid that exhibits remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractinia species, H. symbiolongicarpus and H. echinata , placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpus and identified cell type markers for all major cell types, including key i-cell markers. Orthology analyses based on the markers revealed that Hydractinia 's i-cells are highly enriched in genes that are widely shared amongst animals, a striking finding given that Hydractinia has a higher proportion of phylum-specific genes than any of the other 41 animals in our orthology analysis. These results indicate that Hydractinia 's stem cells and early progenitor cells may use a toolkit shared with all animals, making it a promising model organism for future exploration of stem cell biology and regenerative medicine. The genomic and transcriptomic resources for Hydractinia presented here will enable further studies of their regenerative capacity, colonial morphology, and ability to distinguish self from non-self. Competing Interests: Competing interests: The authors declare no competing financial interests.
Databáze:	MEDLINE
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