Comparison of exoskeleton microbial communities of co-occurring native and invasive crayfish species.

Autor: Grbin D; Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: dorotea.polovic@biol.pmf.hr., Geček S; Ruđer Bošković Institute, Bijenička cesta 54, 10000 Zagreb, Croatia. Electronic address: Suncana.Gecek@irb.hr., Miljanović A; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: andela.miljanovic@pbf.unizg.hr., Pavić D; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: dora.pavic@pbf.unizg.hr., Hudina S; Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia. Electronic address: sandra.hudina@biol.pmf.hr., Žučko J; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: jurica.zucko@pbf.unizg.hr., Rieder J; Institute for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland; Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012 Bern, Switzerland; Swiss Institute of Bioinformatics, Quartier Sorge - Batiment Amphipole, 1015 Lausanne, Switzerland. Electronic address: jessica.rieder@unibe.ch., Pisano SRR; Institute for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland. Electronic address: simone.pisano@unibe.ch., Adrian-Kalchhauser I; Institute for Fish and Wildlife Health, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland. Electronic address: irene.adrian-kalchhauser@unibe.ch., Bielen A; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. Electronic address: ana.bielen@pbf.unizg.hr.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2023 Nov; Vol. 201, pp. 107996. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1016/j.jip.2023.107996
Abstrakt: Host-associated microbial communities are an important determinant of individual fitness and have recently been highlighted as one of the factors influencing the success of invasive species. Invasive hosts introduce their microbes into the new environment, and then both the host and its associated microbes enter into a series of interactions with the native macroscopic and microscopic biota. As these processes are largely unexplored, we aimed to compare the exoskeletal microbial communities of co-occurring and phylogenetically related crayfish: the native narrow-clawed crayfish Pontastacus leptodactylus and the invasive signal crayfish Pacifastacus leniusculus from the recently invaded Korana River, Croatia. The results of high-throughput 16S rRNA sequencing showed that the exoskeletal microbiome of both species is very diverse, significantly influenced by the local environment and dominated by low abundance bacterial families from the phylum Proteobacteria. Furthermore, the exoskeletal microbiomes of the crayfish species differed significantly in the composition and abundance of Amplicon Sequence Variants (ASVs), suggesting that they are to some extent shaped by species-specific intrinsic factors, despite sharing a common habitat. However, over 95% of the bacterial genera associated with the exoskeleton were detected in the exoskeleton samples of both native and invasive crayfish. We paid particular attention to two known crayfish pathogens, Aphanomyces astaci and Saprolegnia parasitica, and find that both species carry low amounts of both pathogens. On the side, we find that a non-standard ddPCR protocol outperforms standard qPCR test for A. astaci under low concentration conditions. Taken together, our results indicate the possibility of bidirectional mixing and homogenisation of exoskeleton microbiome. As such, they can serve as a baseline in future detangling of the processes that act together to shape the microbiomes of co-occuring native and invasive congeners during biological invasions.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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