| Autor: |
Khavinson VK; Saint-Petersburg Institute of Bioregulation and Gerontology, 3 Dynamo pr., St. Petersburg 197110, Russian Federation, e-mail: ibg@gerontology.ru.; I.P.Pavlov Institute of Physiology of RAS, 6 Makarova emb., St. Petersburg 199034, Russian Federation., Linkova NS; Saint-Petersburg Institute of Bioregulation and Gerontology, 3 Dynamo pr., St. Petersburg 197110, Russian Federation, e-mail: ibg@gerontology.ru.; Belgorod State National Research University, 85 Pobeda str., Belgorod 308015, Russian Federation.; Saint-Petersburg Research Institute of Phthisiopulmonology, 2-4 Lygovsky pr., St. Petersburg 191036, Russian Federation., Ashapkin VV; A.N.Belozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye gori, Moscow 119992, Russian Federation., Shilovsky GA; Faculty of Biology, M.V.Lomonosov Moscow State University, 1 Leninskiye gori, Moscow 119234, Russian Federation., Borushko NV; B.P.Konstantinov Petersburg Nuclear Physics Institute, National Research Center «Kurchatov Institute», 1 mkr. Orlova rosha, Gatchina 188300, Leningradskaya area, Russian Federation., Petukhov MG; B.P.Konstantinov Petersburg Nuclear Physics Institute, National Research Center «Kurchatov Institute», 1 mkr. Orlova rosha, Gatchina 188300, Leningradskaya area, Russian Federation., Vanuyshin BF; A.N.Belozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, 1 Leninskiye gori, Moscow 119992, Russian Federation. |
| Abstrakt: |
It was shown that KE peptide (Lys-Glu, vilon) has immunomodulatory, oncostatic and geroprotective effects. The aim of this work is to evaluate the effect of the KE peptide on gene expression and protein synthesis of SIRT1, PARP1, PARP2 during aging of human mesenchymal stem cells (MSC). The KE peptide increased gene expression and synthesis of the SIRT1 protein in «young» MSCs by 6 and 8,2 times, respectively. The KE peptide reduced gene expression and PARP1 protein synthesis during MSC aging by 2,1 and 5,3 times, respectively; and also reduced gene expression and PARP2 protein synthesis by 2,1 and 4,7 times, respectively. According to molecular modeling data, the KE peptide can interact with the GCGG sequence of double-stranded DNA (dsDNA) in the classical B-form and with the GGGC sequence of the curved dsDNA nucleosome. The indicated dsDNA sequences were found in the promoters of the human SIRT1, PARP1, PARP2 genes. Thus, the KE peptide regulates gene expression and synthesis of SIRT1, PARP1, PARP2 proteins in human mesenchymal stem cells during replicative ageing, which underlies the biological activity and geroprotective effect of this peptide. |
