Impact of Extracellular DNA on Architectural Parameters of Leptospira biflexa Biofilm.

Autor: Gomes T; Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil.; Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901 Brazil.; National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution (INCT IN-TREE), Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil., Ribeiro PS; Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil.; Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais 31270-901 Brazil.; National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution (INCT IN-TREE), Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil., Carvalho NB; Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil.; National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution (INCT IN-TREE), Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil., El-Hani CN; Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil.; National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution (INCT IN-TREE), Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil., Figueira CP; Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Bahia 40296-710 Brazil., Ristow P; Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil.; National Institute of Science and Technology in Interdisciplinary and Transdisciplinary Studies in Ecology and Evolution (INCT IN-TREE), Institute of Biology, Federal University of Bahia, Salvador, Bahia 40170-115 Brazil.; Gonçalo Moniz Institute, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Bahia 40296-710 Brazil.
Jazyk: angličtina
Zdroj: Indian journal of microbiology [Indian J Microbiol] 2023 Sep; Vol. 63 (3), pp. 373-379. Date of Electronic Publication: 2023 Sep 02.
DOI: 10.1007/s12088-023-01085-6
Abstrakt: Extracellular DNA (eDNA) is a major component of bacterial biofilms. In this study, we performed a three-dimensional analysis of Leptospira biofilm using advanced imaging by confocal laser scanning microscopy (CLSM) and multi-parameter analysis by COMSTAT 2 software, with quantification of Leptospira and eDNA fluorescence. To investigate the role of eDNA in Leptospira biofilm, we treated Leptospira biflexa biofilms with DNase I enzyme (DNase), which digested eDNA, and compared DNase treated biofilms and controls. There was a significant reduction of the biomass of biofilms treated with DNase, by spectrophotometry and COMSTAT analysis. The multiparameter analysis evidenced for DNase-treated biofilms a significant decrease in the surface area and the average thickness; opposing to a significant augmentation of the surface/biovolume ratio and the roughness coefficient (Ra*), when compared to controls. We analyzed the parameters of DNase-treated biofilms by Pearson's correlation coefficient and found significant positive correlations between biomass and average thickness; biomass and surface area; surface area and average thickness. On the other hand, there were significant negative correlations between Ra* and biomass; Ra* and average thickness; Ra* and surface area. These findings suggest that eDNA digestion results in biofilm instability and alteration of the three-dimensional architecture, justifying the negative correlation between Ra* and the above-mentioned parameters. In conclusion, our study showed that eDNA digestion produced a massive structural loss, instability, and dramatic changes in the three-dimensional architecture of Leptospira biflexa biofilm. These findings contribute to a better understanding of the role of eDNA and highlight the importance of eDNA as a key component in Leptospira biofilms.
Competing Interests: Conflict of interestThe authors declare that they have no conflicts of interest.
(© Association of Microbiologists of India 2023.)
Databáze: MEDLINE
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