Effect of Yarrowia lipolytica yeast biomass with increased kynurenic acid content on selected metabolic indicators in mice.

Autor:	Matusiewicz M; Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland., Wróbel-Kwiatkowska M; Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland., Niemiec T; Department of Animal Nutrition, Institute of Animal Sciences, Warsaw University of Life Sciences, Warsaw, Poland., Świderek W; Department of Animal Genetics and Conservation, Institute of Animal Sciences, Warsaw University of Life Sciences, Warsaw, Poland., Kosieradzka I; Department of Animal Nutrition, Institute of Animal Sciences, Warsaw University of Life Sciences, Warsaw, Poland., Rosińska A; Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland., Niwińska A; Department of Large Animal Diseases and Clinic, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland., Rakicka-Pustułka M; Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland., Kocki T; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland., Rymowicz W; Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland., Turski WA; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, Lublin, Poland.
Jazyk:	angličtina
Zdroj:	PeerJ [PeerJ] 2023 Sep 26; Vol. 11, pp. e15833. Date of Electronic Publication: 2023 Sep 26 (Print Publication: 2023).
DOI:	10.7717/peerj.15833
Abstrakt:	Background: The unconventional yeast species Yarrowia lipolytica is a valuable source of protein and many other nutrients. It can be used to produce hydrolytic enzymes and metabolites, including kynurenic acid (KYNA), an endogenous metabolite of tryptophan with a multidirectional effect on the body. The administration of Y. lipolytica with an increased content of KYNA in the diet may have a beneficial effect on metabolism, which was evaluated in a nutritional experiment on mice. Methods: In the dry biomass of Y. lipolytica S12 enriched in KYNA (high-KYNA yeast) and low-KYNA (control) yeast, the content of KYNA was determined by high-performance liquid chromatography. Then, proximate and amino acid composition and selected indicators of antioxidant status were compared. The effect of 5% high-KYNA yeast content in the diet on the growth, hematological and biochemical indices of blood and the redox status of the liver was determined in a 7-week experiment on adult male mice from an outbred colony derived from A/St, BALB/c, BN/a and C57BL/6J inbred strains. Results: High-KYNA yeast was characterized by a greater concentration of KYNA than low-KYNA yeast (0.80 ± 0.08 vs . 0.29 ± 0.01 g/kg dry matter), lower content of crude protein with a less favorable amino acid composition and minerals, higher level of crude fiber and fat and lower ferric-reducing antioxidant power, concentration of phenols and glutathione. Consumption of the high-KYNA yeast diet did not affect the cumulative body weight gain per cage, cumulative food intake per cage and protein efficiency ratio compared to the control diet. A trend towards lower mean corpuscular volume and hematocrit, higher mean corpuscular hemoglobin concentration and lower serum total protein and globulins was observed, increased serum total cholesterol and urea were noted. Its ingestion resulted in a trend towards greater ferric-reducing antioxidant power in the liver and did not affect the degree of liver lipid and protein oxidation. Conclusions: The improvement of the quality of Y. lipolytica yeast biomass with increased content of KYNA, including its antioxidant potential, would be affected by the preserved level of protein and unchanged amino acid profile. It will be worth investigating the effect of such optimized yeast on model animals, including animals with metabolic diseases. Competing Interests: The authors declare that they have no competing interests. (© 2023 Matusiewicz et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu