Biofilm formation initiating rotifer-specific biopolymer and its predicted components.

Autor: Datki Z; Micro-In Vivo Biomolecule Research Laboratory, Competence Centre of the Life Sciences Cluster of the Centre of Excellence for Interdisciplinary Research, Development and Innovation of the University of Szeged. Dugonics ter 13. H-6720, Szeged, Hungary. Electronic address: datki.zsolt.laszlo@szte.hu., Darula Z; Single Cell Omics Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine, Szeged, Hungary; Proteomics Research Group, Core Facilities, Biological Research Centre, ELKH, Szeged, Hungary., Vedelek V; Department of Genetics, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726, Hungary., Hunyadi-Gulyas E; Proteomics Research Group, Core Facilities, Biological Research Centre, ELKH, Szeged, Hungary., Dingmann BJ; Department of Math Science and Technology, University of Minnesota Crookston, 2900 University Avenue, Crookston, MN 56716, United States of America., Vedelek B; Department of Genetics, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726, Hungary., Kalman J; Department of Psychiatry, Albert Szent-Gyorgyi Medical School, University of Szeged, Koranyi Fasor 8-10, H-6725 Szeged, Hungary., Urban P; Szentagothai Research Center, Genomic and Bioinformatic Core Facility, Pecs, Hungary., Gyenesei A; Szentagothai Research Center, Genomic and Bioinformatic Core Facility, Pecs, Hungary., Galik-Olah Z; Micro-In Vivo Biomolecule Research Laboratory, Competence Centre of the Life Sciences Cluster of the Centre of Excellence for Interdisciplinary Research, Development and Innovation of the University of Szeged. Dugonics ter 13. H-6720, Szeged, Hungary., Galik B; Szentagothai Research Center, Genomic and Bioinformatic Core Facility, Pecs, Hungary., Sinka R; Department of Genetics, Faculty of Science and Informatics, University of Szeged, Kozep fasor 52, H-6726, Hungary.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Dec 31; Vol. 253 (Pt 5), pp. 127157. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1016/j.ijbiomac.2023.127157
Abstrakt: The rotifer-specific biopolymer, namely Rotimer, is a recently discovered group of the biomolecule family. Rotimer has an active role in the biofilm formation initiated by rotifers (e.g., Euchlanis dilatata or Adineta vaga) or in the female-male sexual interaction of monogononts. To understand the Ca 2+ - and polarity-dependent formation of this multifunctional viscoelastic material, it is essential to explore its molecular composition. The investigation of the rotifer-enhanced biofilm and Rotimer-inductor conglomerate (RIC) formation yielded several protein candidates to predict the Rotimer-specific main components. The exudate of E. dilatata males was primarily applied from different biopolimer-containing samples (biofilm or RIC). The advantage of males over females lies in their degenerated digestive system and simple anatomy. Thus, their exudate is less contaminated with food and endosymbiont elements. The sequenced and annotated genome and transcriptome of this species opened the way for identifying Rotimer proteins by mass spectrometry. The predicted rotifer-biopolymer forming components are SCO-spondins and 14-3-3 protein. The characteristics of Rotimer are similar to Reissner's fiber, which is found in the central nervous system of vertebrates and is mainly formed from SCO-spondins. This molecular information serves as a starting point for its interdisciplinary investigation and application in biotechnology, biomedicine, or neurodegeneration-related drug development.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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