Origin of breath isoprene in humans is revealed via multi-omic investigations.

Autor: Sukul P; Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Dept. of Anesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany. pritam.sukul@uni-rostock.de., Richter A; Department of Medicine, Clinic III - Hematology, Oncology, Palliative Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057, Rostock, Germany., Junghanss C; Department of Medicine, Clinic III - Hematology, Oncology, Palliative Medicine, Rostock University Medical Center, Ernst-Heydemann-Strasse 6, 18057, Rostock, Germany., Schubert JK; Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Dept. of Anesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany., Miekisch W; Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Dept. of Anesthesiology, Intensive Care Medicine and Pain Therapy, University Medicine Rostock, Schillingallee 35, 18057, Rostock, Germany.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2023 Sep 30; Vol. 6 (1), pp. 999. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1038/s42003-023-05384-y
Abstrakt: Plants, animals and humans metabolically produce volatile isoprene (C 5 H 8 ). Humans continuously exhale isoprene and exhaled concentrations differ under various physio-metabolic and pathophysiological conditions. Yet unknown metabolic origin hinders isoprene to reach clinical practice as a biomarker. Screening 2000 individuals from consecutive mass-spectrometric studies, we herein identify five healthy German adults without exhaled isoprene. Whole exome sequencing in these adults reveals only one shared homozygous (European prevalence: <1%) IDI2 stop-gain mutation, which causes losses of enzyme active site and Mg 2+ -cofactor binding sites. Consequently, the conversion of isopentenyl diphosphate to dimethylallyl diphosphate (DMAPP) as part of the cholesterol metabolism is prevented in these adults. Targeted sequencing depicts that the IDI2 rs1044261 variant (p.Trp144Stop) is heterozygous in isoprene deficient blood-relatives and absent in unrelated isoprene normal adults. Wild-type IDI1 and cholesterol metabolism related serological parameters are normal in all adults. IDI2 determines isoprene production as only DMAPP sources isoprene and unlike plants, humans lack isoprene synthase and its enzyme homologue. Human IDI2 is expressed only in skeletal-myocellular peroxisomes and instant spikes in isoprene exhalation during muscle activity underpins its origin from muscular lipolytic cholesterol metabolism. Our findings translate isoprene as a clinically interpretable breath biomarker towards potential applications in human medicine.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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