Systemic and local responses of cytokines and tissue histology following intramammary lipopolysaccharide challenge in dairy cows.

Autor:	Choudhary RK; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT 05405., Olszanski L; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT 05405., McFadden TB; Division of Animal Sciences, University of Missouri, Columbia, MO 65211., Lalonde C; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT 05405., Spitzer A; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT 05405., Shangraw EM; Division of Animal Sciences, University of Missouri, Columbia, MO 65211., Rodrigues RO; Division of Animal Sciences, University of Missouri, Columbia, MO 65211., Zhao FQ; Department of Animal and Veterinary Sciences, University of Vermont, Burlington, VT 05405. Electronic address: fzhao@uvm.edu.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2024 Feb; Vol. 107 (2), pp. 1299-1310. Date of Electronic Publication: 2023 Sep 29.
DOI:	10.3168/jds.2023-23543
Abstrakt:	During bovine mastitis, immune responses include the release of cytokines and the recruitment of leukocytes, resulting in profound structural and functional changes in the mammary gland. Our aims were to delineate systemic and local cytokine responses and to quantify histological changes in the mammary tissue of lactating cows after acute intramammary lipopolysaccharide (LPS) challenge. Ten multiparous dairy cows were paired to either treatment (TRT) or control (CON) groups. For TRT cows, one side of the udder was randomly assigned to receive treatment with LPS (50 µg in 10 mL of saline, TL) into both the front and rear quarters; the contralateral quarters received saline (10 mL). Udder-halves of CON cows were similarly assigned randomly to receive either saline (10 mL, CS) or no infusion (untreated). Temporal changes in the concentrations of 15 cytokines in the blood (0, 3, 6, 12, and 24 h relative to the LPS infusion) and in mammary tissue (0, 3, and 12 h) were determined, as were concomitant changes in mammary histology. The cytokines IL-6, IL-10, MCP-1, and MIP-1β showed a systemic response as their concentrations were significantly different in the plasma of TRT cows as compared with CON cows after LPS challenge. The cytokines IL-1α, IL-1β, IL-6, IL-8, IL-17A, IL-36RA, IP-10, MCP-1, MIP-1α, MIP-1β, TNF-α, and VEGF-A showed a local response in TL glands, and 8 cytokines, IL-1β, IL-6, IL-10, IL-17A, IL-36RA, IP-10, MIP-1β, and VEGF-A showed systemic changes in the nonchallenged mammary glands adjacent to LPS-infused glands. Endotoxin challenge evoked changes in the histology of mammary tissue that included a 5.2- and 7.2-fold increases in the number of neutrophils in alveolar lumens at 3 h and 12 h, respectively. In summary, LPS challenge induced specific local and systemic responses in cytokine induction and elicited neutrophil infiltration in bovine mammary tissue. (The Authors. Published by Elsevier Inc. and Fass Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).)
Databáze:	MEDLINE
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