Molecular insights into RmcA-mediated c-di-GMP consumption: Linking redox potential to biofilm morphogenesis in Pseudomonas aeruginosa.

Autor: Scribani Rossi C; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Eckartt K; Department of Biological Sciences, Columbia University, New York, USA., Scarchilli E; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Angeli S; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Price-Whelan A; Department of Biological Sciences, Columbia University, New York, USA., Di Matteo A; CNR Institute of Molecular Biology and Pathology, I-00185 Rome, Italy., Chevreuil M; Plate-forme de Biophysique Moléculaire, Institut Pasteur, UMR 3528 CNRS, Paris, France., Raynal B; Plate-forme de Biophysique Moléculaire, Institut Pasteur, UMR 3528 CNRS, Paris, France., Arcovito A; Dipartimento di Scienze Biotecnologiche Di Base, Cliniche Intensivologiche e Perioperatorie Università Cattolica Del Sacro Cuore, Roma, Italy; Fondazione Policlinico Universitario A. Gemelli - IRCCS, Rome, Italy., Giacon N; Dipartimento di Scienze Biotecnologiche Di Base, Cliniche Intensivologiche e Perioperatorie Università Cattolica Del Sacro Cuore, Roma, Italy., Fiorentino F; Department of Drug Chemistry and Technologies, Sapienza University of Rome, Rome, Italy., Rotili D; Department of Drug Chemistry and Technologies, Sapienza University of Rome, Rome, Italy., Mai A; Department of Drug Chemistry and Technologies, Sapienza University of Rome, Rome, Italy., Espinosa-Urgel M; Department of Biotechnology and Environmental Protection. Estación Experimental del Zaidin, CSIC, Granada, Spain., Cutruzzolà F; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Dietrich LEP; Department of Biological Sciences, Columbia University, New York, USA., Paone A; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Paiardini A; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Rinaldo S; Laboratory affiliated to Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy. Electronic address: serena.rinaldo@uniroma1.it.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2023 Dec; Vol. 277, pp. 127498. Date of Electronic Publication: 2023 Sep 15.
DOI: 10.1016/j.micres.2023.127498
Abstrakt: The ability of many bacteria to form biofilms contributes to their resilience and makes infections more difficult to treat. Biofilm growth leads to the formation of internal oxygen gradients, creating hypoxic subzones where cellular reducing power accumulates, and metabolic activities can be limited. The pathogen Pseudomonas aeruginosa counteracts the redox imbalance in the hypoxic biofilm subzones by producing redox-active electron shuttles (phenazines) and by secreting extracellular matrix, leading to an increased surface area-to-volume ratio, which favors gas exchange. Matrix production is regulated by the second messenger bis-(3',5')-cyclic-dimeric-guanosine monophosphate (c-di-GMP) in response to different environmental cues. RmcA (Redox modulator of c-di-GMP) from P. aeruginosa is a multidomain phosphodiesterase (PDE) that modulates c-di-GMP levels in response to phenazine availability. RmcA can also sense the fermentable carbon source arginine via a periplasmic domain, which is linked via a transmembrane domain to four cytoplasmic Per-Arnt-Sim (PAS) domains followed by a diguanylate cyclase (DGC) and a PDE domain. The biochemical characterization of the cytoplasmic portion of RmcA reported in this work shows that the PAS domain adjacent to the catalytic domain tunes RmcA PDE activity in a redox-dependent manner, by differentially controlling protein conformation in response to FAD or FADH2. This redox-dependent mechanism likely links the redox state of phenazines (via FAD/FADH2 ratio) to matrix production as indicated by a hyperwrinkling phenotype in a macrocolony biofilm assay. This study provides insights into the role of RmcA in transducing cellular redox information into a structural response of the biofilm at the population level. Conditions of resource (i.e. oxygen and nutrient) limitation arise during chronic infection, affecting the cellular redox state and promoting antibiotic tolerance. An understanding of the molecular linkages between condition sensing and biofilm structure is therefore of crucial importance from both biological and engineering standpoints.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier GmbH.. All rights reserved.)
Databáze: MEDLINE
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