N 6 -Methyladenosine (m 6 A) Methylation Is Associated with the Immune Microenvironments in Acute Intracerebral Hemorrhage (ICH).

Autor: Yang H; Department of Neurology, The First Rehabilitation Hospital of Shanghai, School of Medicine, Tongji University, Shanghai, China., Xie C; Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Wu YF; Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Cheng Y; Department of Neurology, The First Rehabilitation Hospital of Shanghai, School of Medicine, Tongji University, Shanghai, China., Zhu DS; Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China., Guan YT; Department of Neurology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. yangtaiguan@sina.com.; Department of Neurology, Shanghai Yangzhi Rehabilitation Hospital, School of Medicine, Tongji University, Shanghai, China. yangtaiguan@sina.com.
Jazyk: angličtina
Zdroj: Molecular neurobiology [Mol Neurobiol] 2024 Mar; Vol. 61 (3), pp. 1781-1793. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1007/s12035-023-03643-x
Abstrakt: Researchers have recently found that N 6 -methyladenosine (m 6 A) is a type of internal posttranscriptional modification that is essential in mammalian mRNA. However, the features of m 6 A RNA methylation in acute intracerebral hemorrhage (ICH) remain unknown. To explore differential methylations and to discover their functions in acute ICH patients, we recruited three acute ICH patients, three healthy controls, and an additional three patients and healthy controls for validation. The m 6 A methylation levels in blood samples from the two groups were determined by ultrahigh-performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS). Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was employed to identify differences in m 6 A modification, and the differentially expressed m 6 A-modified genes were confirmed by MeRIP-qPCR. We found no significant differences in the total m 6 A levels between the two groups but observed differential methylation peaks. Compared with the control group, the coding genes showing increased methylation following acute ICH were mostly involved in processes connected with osteoclast differentiation, the neurotrophin signaling pathway, and the spliceosome, whereas genes with reduced m 6 A modification levels after acute ICH were found to be involved in the B-cell and T-cell receptor signaling pathways. These results reveal that differentially m 6 A-modified genes may influence the immune microenvironments in acute ICH.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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