Association of HOTAIR gene rs920778 (C > T) and rs4759314 (A > G) polymorphism with breast cancer in Egyptian women.

Autor: Anber N; Emergency Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt. nahlahamedanber@mans.edu.eg., Tarabay MM; Health Insurance Organization (HIO), Mansoura, Mansoura, 35511, Egypt., Elmougy R; Biochemistry Division, Chemistry Department, Faculty of Science, Mansoura University, Mansoura, Egypt., Abdel-Dayem MA; Pharmacology and Toxicology Department, Faculty of Pharmacy, Horus University, New Damietta City, Egypt., Elbendary EY; Clinical Nutrition Department, College of Applied Medical Sciences, Jazan University, Jazan, Kingdom of Saudi Arabia.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2023 Nov; Vol. 50 (11), pp. 9153-9163. Date of Electronic Publication: 2023 Sep 30.
DOI: 10.1007/s11033-023-08725-6
Abstrakt: Background: Hox transcript antisense RNA (HOTAIR) is considered an oncogene associated with the initiation and progression of many malignancies. Previous studies have examined the connection between HOTAIR SNPs rs4759314 and rs920778 for breast cancer (BC), getting variable results in multiple ethnicities. Therefore, this study was designed to evaluate the connection between these two SNPs and disease vulnerability, clinic-laboratory, and hormonal parameters, featuring status associations with the BC risk in an Egyptian woman sample.
Methods and Results: In this case-control study, DNA was taken from the blood of 100 BC patients and 100 unrelated healthy Egyptian females. The characterization of rs4759314 was genotyped using the T-ARMS-PCR method and rs920778 using the SNP-RFLP technique for all participants. The frequency of the rs4759314 A > G variation revealed a statistically significant increase in BC risk with dominant (p = 0.013, OR = 1.592, 95% Cl = 1.105-2.293), co-dominant (p = 0.006, OR = 2.314, 95%Cl = 1.278-4.191) and overdominant (p = 0.002, OR = 2.571, 95% Cl = 1.430-4.624) genetic models. On the other hand, the rs920778 C > T polymorphism was not significantly associated with BC. ER/PR positivity with HER2 negativity was significantly associated with the AA genotype compared to the AG genotype. Otherwise, no significant associations between the two SNPs and clinical stage or hormonal features could be found. In conclusion, the rs4759314 A > G SNP in the HOTAIR gene is strongly associated with BC, which might warrant its determination among affected families for prevention and early treatment.
(© 2023. The Author(s).)
Databáze: MEDLINE
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