Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production.

Autor:	Hegde TR; Department of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL, USA., Rufus OO; Department of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL, USA. ookocharufus@hawk.iit.edu., Lee J; Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk, South Korea., Hong SH; Department of Chemical and Biological Engineering, Illinois Institute of Technology, Chicago, IL, USA.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2720, pp. 3-16.
DOI:	10.1007/978-1-0716-3469-1_1
Abstrakt:	Cell-free protein synthesis provides a flexible platform for the production of difficult-to-express proteins, because maintaining cell viability is unnecessary. The antimicrobial proteins known as bacteriocins have great potential for development as antibiotic alternatives. Here, we describe detailed protocols for producing and characterizing colicins-antimicrobial proteins that are produced by Escherichia coli hosts and inactivate nonhost E. coli strains. Active colicins can be produced with lysates containing molecular chaperones and coproduction of immunity proteins in cell-free protein synthesis reactions. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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