Alpha-Fetoprotein as a Factor of Differentiation and Functional Activity of Myeloid-Derived Suppressor Cells.

Autor: Shardina KY; Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia. shardinak@gmail.com., Zamorina SA; Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia.; Perm State National Research University, Perm, Russia., Timganova VP; Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia., Bochkova MS; Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia.; Perm State National Research University, Perm, Russia., Uzhviyuk SV; Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia., Chereshnev VA; Institute of Ecology and Genetics of Microorganisms, Ural Division of the Russian Academy of Sciences - Branch of Perm Federal Research Center, Ural Division of the Russian Academy of Sciences, Perm, Russia.; Perm State National Research University, Perm, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Aug; Vol. 175 (4), pp. 535-543. Date of Electronic Publication: 2023 Sep 29.
DOI: 10.1007/s10517-023-05901-3
Abstrakt: We studied the role of alpha-fetoprotein (AFP) in regulation of differentiation and functional activity of human myeloid-derived suppressor cells (MDSC) in vitro. To obtain MDSC, CD11b + cells were isolated from the peripheral blood of healthy donors followed by cytokine induction (IL-1β+GM-CSF) into the MDSC phenotype. The cell functions were assessed by the expression of indoleamine 2,3-dioxygenase (IDO) and arginase-1 (Arg1) and cytokine profile of the cell cultures. Native AFP did not affect the total number of MDSC and the percentage of polymorphonuclear MDSC (PMN-MDSC), but increased the number of monocytic MDSC (M-MDSC). AFP did not change the expression of Arg1, but in low concentrations (10 and 50 U/ml) increased the number of IDO-containing cells. AFP modulated the cytokine profile of CD11b + cells: it reliably decreased the level of IL-19 (50 and100 U/ml) and showed a tendency to decrease the levels of IL-34, MMP-2, sCD163, CHI3L1, OPN and to increase the levels of IL-29, IL-32, APRIL, PTX3, and sTNF-R1. Thus, we have demonstrated a regulatory effect of native AFP at the level of MDSC generated from CD11b + cells under conditions of cytokine induction in vitro.
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Databáze: MEDLINE
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