An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration.

Autor: Romero-Torrecilla JA; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain., Lamo-Espinosa JM; Department of Orthopedic Surgery and Traumatology, Clínica Universidad de Navarra, Pamplona, Spain.; Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain., Ripalda-Cemboráin P; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain.; Department of Orthopedic Surgery and Traumatology, Clínica Universidad de Navarra, Pamplona, Spain.; Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain., López-Martínez T; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain.; Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain., Abizanda G; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain., Riera-Álvarez L; Department of Orthopedic Surgery and Traumatology, Clínica Universidad de Navarra, Pamplona, Spain., de Galarreta-Moriones SR; Tecnun-School of Engineering, Universidad de Navarra, San Sebastian, Spain., López-Barberena A; Tecnun-School of Engineering, Universidad de Navarra, San Sebastian, Spain., Rodríguez-Flórez N; Tecnun-School of Engineering, Universidad de Navarra, San Sebastian, Spain.; IKERBASQUE, Basque Foundation for Science, Bilbao, Spain., Elizalde R; Tecnun-School of Engineering, Universidad de Navarra, San Sebastian, Spain., Jayawarna V; Center for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, Glasgow, United Kingdom., Valdés-Fernández J; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain., de Anleo ME; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain., Childs P; Department of Biomedical Engineering, University of Strathclyde, Glasgow, United Kingdom., de Juan-Pardo E; T3mPLATE, Harry Perkins Institute of Medical Research, Queen Elizabeth II Medical Centre and the UWA Centre for Medical Research, The University of Western Australia, Perth, Australia., Salmeron-Sanchez M; Center for the Cellular Microenvironment, James Watt School of Engineering, University of Glasgow, Glasgow, United Kingdom., Prósper F; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain.; Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain.; Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Pamplona, Spain.; Department of Hematology, Clínica Universidad de Navarra, Pamplona, Spain., Muiños-López E; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain. emuinos@unav.es.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain. emuinos@unav.es.; Department of Orthopedic Surgery and Traumatology, Clínica Universidad de Navarra, Pamplona, Spain. emuinos@unav.es.; Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain. emuinos@unav.es., Granero-Moltó F; Cell Therapy Area, Clínica Universidad de Navarra, Pamplona, Spain. fgranero@unav.es.; Biomedical Engineering Program, Centro de Investigación Médica Aplicada (CIMA), Pamplona, Spain. fgranero@unav.es.; Department of Orthopedic Surgery and Traumatology, Clínica Universidad de Navarra, Pamplona, Spain. fgranero@unav.es.; Instituto de Investigaciones Sanitarias de Navarra (IdiSNA), Pamplona, Spain. fgranero@unav.es.
Jazyk: angličtina
Zdroj: NPJ Regenerative medicine [NPJ Regen Med] 2023 Sep 29; Vol. 8 (1), pp. 54. Date of Electronic Publication: 2023 Sep 29.
DOI: 10.1038/s41536-023-00330-2
Abstrakt: During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (ethyl acrylate) coating of the PCL membrane allowed functionalization, mediated by fibronectin and low dose recombinant human BMP-2 (rhBMP-2) (10-25 μg/ml), resulting in efficient, sustained osteoinduction in vitro. In vivo, rhBMP-2 functionalized mimetic periosteum demonstrated regenerative potential in the treatment of rat critical-size femoral defects with highly efficient healing and functional recovery (80%-93%). Mimetic periosteum has also proven to be efficient for cell delivery, as observed through the migration of transplanted periosteum-derived mesenchymal cells to the bone defect and their survival. Ultimately, mimetic periosteum demonstrated its ability to deliver key stem cells and morphogens to an injured site, exposing a therapeutic and translational potential in vivo when combined with unprecedentedly low rhBMP-2 doses.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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