Genomic epidemiology reveals multidrug resistant plasmid spread between Vibrio cholerae lineages in Yemen.

Autor: Lassalle F; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK. florent.lassalle@sanger.ac.uk., Al-Shalali S; Faculty of Science, Sana'a University, Sana'a, Yemen., Al-Hakimi M; Faculty of Science, Sana'a University, Sana'a, Yemen., Njamkepo E; Institut Pasteur, Université Paris Cité, Unité des Bactéries pathogènes entériques, Paris, France., Bashir IM; WHO Yemen country office, Sana'a, Yemen., Dorman MJ; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK.; Churchill College, Cambridge, UK., Rauzier J; Institut Pasteur, Université Paris Cité, Unité des Bactéries pathogènes entériques, Paris, France., Blackwell GA; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK.; EMBL-EBI, Hinxton, UK., Taylor-Brown A; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK., Beale MA; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK., Cazares A; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK., Al-Somainy AA; National Centre of Public Health Laboratories, Sana'a, Yemen., Al-Mahbashi A; Faculty of Science, Sana'a University, Sana'a, Yemen., Almoayed K; National Centre of Public Health Laboratories, Sana'a, Yemen., Aldawla M; Ministry of Public Health, Infection Control Unit, Sana'a, Yemen., Al-Harazi A; National Centre of Public Health Laboratories, Sana'a, Yemen., Quilici ML; Institut Pasteur, Université Paris Cité, Unité des Bactéries pathogènes entériques, Paris, France., Weill FX; Institut Pasteur, Université Paris Cité, Unité des Bactéries pathogènes entériques, Paris, France., Dhabaan G; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada. ghulam.dhabaan@utoronto.ca., Thomson NR; Parasites and Microbes Programme, Wellcome Sanger Institute, Hinxton, UK. nrt@sanger.ac.uk.; London School of Hygiene and Tropical Medicine, London, UK. nrt@sanger.ac.uk.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2023 Oct; Vol. 8 (10), pp. 1787-1798. Date of Electronic Publication: 2023 Sep 28.
DOI: 10.1038/s41564-023-01472-1
Abstrakt: Since 2016, Yemen has been experiencing the largest cholera outbreak in modern history. Multidrug resistance (MDR) emerged among Vibrio cholerae isolates from cholera patients in 2018. Here, to characterize circulating genotypes, we analysed 260 isolates sampled in Yemen between 2018 and 2019. Eighty-four percent of V. cholerae isolates were serogroup O1 belonging to the seventh pandemic El Tor (7PET) lineage, sub-lineage T13, whereas 16% were non-toxigenic, from divergent non-7PET lineages. Treatment of severe cholera with macrolides between 2016 and 2019 coincided with the emergence and dominance of T13 subclones carrying an incompatibility type C (IncC) plasmid harbouring an MDR pseudo-compound transposon. MDR plasmid detection also in endemic non-7PET V. cholerae lineages suggested genetic exchange with 7PET epidemic strains. Stable co-occurrence of the IncC plasmid with the SXT family of integrative and conjugative element in the 7PET background has major implications for cholera control, highlighting the importance of genomic epidemiological surveillance to limit MDR spread.
(© 2023. The Author(s).)
Databáze: MEDLINE
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