Simple method for quantification of metal-based particles in biopsy samples of patients with long bone implants - Pilot study.

Autor: Olšovská E; Nanotechnology Centre, CEET, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic; Faculty of Material Science and Technology, Center for Advanced Innovation Technologies, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic. Electronic address: eva.olsovska@vsb.cz., Čabanová K; Faculty of Material Science and Technology, Center for Advanced Innovation Technologies, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic; Faculty of Mining and Geology, VSB-Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic., Motyka O; Nanotechnology Centre, CEET, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic; Faculty of Mining and Geology, VSB-Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic., Kryštofová HB; Faculty of Material Science and Technology, Center for Advanced Innovation Technologies, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic; Institute of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava and Faculty of Medicine, 17. listopadu 1790/5, Ostrava-Poruba 708 52, Czech Republic; Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, Syllabova 19, Ostrava 703 00, Czech Republic., Matějková P; Faculty of Material Science and Technology, Center for Advanced Innovation Technologies, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic., Voves J; Department of Trauma Surgery, University Hospital Ostrava, 17. listopadu 1790/5, Ostrava-Poruba 708 52, Czech Republic; Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, Syllabova 19, Ostrava 703 00, Czech Republic., Židlík V; Institute of Molecular and Clinical Pathology and Medical Genetics, University Hospital Ostrava and Faculty of Medicine, 17. listopadu 1790/5, Ostrava-Poruba 708 52, Czech Republic; Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, Syllabova 19, Ostrava 703 00, Czech Republic., Madeja R; Department of Trauma Surgery, University Hospital Ostrava, 17. listopadu 1790/5, Ostrava-Poruba 708 52, Czech Republic; Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, Syllabova 19, Ostrava 703 00, Czech Republic., Demel J; Department of Trauma Surgery, University Hospital Ostrava, 17. listopadu 1790/5, Ostrava-Poruba 708 52, Czech Republic; Institute of Emergency, Medicine Faculty of Medicine, University of Ostrava, Syllabova 19, Ostrava 703 00, Czech Republic; Faculty of Medicine, Masaryk University, Kamenice 753/5, Brno 625 00, Czech Republic., Halfar J; Faculty of Material Science and Technology, Center for Advanced Innovation Technologies, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic; Faculty of Mining and Geology, VSB-Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic., Kukutschová J; Faculty of Material Science and Technology, Center for Advanced Innovation Technologies, VSB - Technical University of Ostrava, 17. listopadu 2172/15, Ostrava-Poruba 708 00, Czech Republic.
Jazyk: angličtina
Zdroj: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2023 Oct; Vol. 103, pp. 104282. Date of Electronic Publication: 2023 Sep 26.
DOI: 10.1016/j.etap.2023.104282
Abstrakt: The presence of particles fixed in tissue samples due to implant degradation or disintegration plays an important role in post-operative complications. The ability to determine the size, shape, chemical composition and, above all, the number of these particles can be used in many areas of medicine. This study presents a novel, simple metal-based particle detection method using scanning electron microscopy with energy dispersive spectrometer (SEM-EDS). The presence of metal particles in biopsy specimens from long bone nail-fixated implants (10 patients with titanium steel nails and 10 patients with stainless steel nails) was studied. The samples were analysed using automated area analysis based on image binarization and brightness to 255 grayscale. The results were supplemented with histological data and statistically analysed. The method based on the software used was found to be accurate and easy to use and, thus, appears to be very suitable for particle detection in similar samples.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: