Nanobody-guided redox and enzymatic functionalization of icosahedral virus particles for enhanced bioelectrocatalysis.

Autor: Kassem R; Université Paris Cité, CNRS, Laboratoire d'Electrochimie Moléculaire, F-75013 Paris, France., Cousin A; Institut de Biologie Moléculaire des Plantes, UPR2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, F-67084 Strasbourg, France., Clesse D; Institut de Biologie Moléculaire des Plantes, UPR2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, F-67084 Strasbourg, France., Poignavent V; Institut de Biologie Moléculaire des Plantes, UPR2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, F-67084 Strasbourg, France., Trolet A; Institut de Biologie Moléculaire des Plantes, UPR2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, F-67084 Strasbourg, France., Ritzenthaler C; Institut de Biologie Moléculaire des Plantes, UPR2357 du Centre National de la Recherche Scientifique, Université de Strasbourg, F-67084 Strasbourg, France. Electronic address: ritzenth@unistra.fr., Michon T; Université de Bordeaux, Biologie du Fruit et Pathologie, INRA UMR 1332, F-33140 Villenave d'Ornon, France. Electronic address: thierry.michon@inrae.fr., Chovin A; Université Paris Cité, CNRS, Laboratoire d'Electrochimie Moléculaire, F-75013 Paris, France. Electronic address: arnaud.chovin@u-paris.fr., Demaille C; Université Paris Cité, CNRS, Laboratoire d'Electrochimie Moléculaire, F-75013 Paris, France. Electronic address: christophe.demaille@u-paris.fr.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2024 Feb; Vol. 155, pp. 108570. Date of Electronic Publication: 2023 Sep 19.
DOI: 10.1016/j.bioelechem.2023.108570
Abstrakt: Icosahedral, 30 nm diameter, grapevine fanleaf virus (GFLV) virus particles are adsorbed onto electrodes and used as nanoscaffolds for the assembly of an integrated glucose oxidizing system, comprising the enzyme pyrroloquinoline quinone-glucose dehydrogenase (PQQ-GDH) and ferrocenylated polyethylene glycol chains (Fc-PEG) as a redox co-substrate. Two different GFLV-specific nanobodies, either fused to the enzyme, or chemically conjugated to Fc-PEG, are used for the regio-selective immunodecoration of the viral particles. A comprehensive kinetic characterization of the enzymatic function of the particles, initially decorated with the enzyme alone shows that simple immobilization on the GFLV capsid has no effect on the kinetic scheme of the enzyme, nor on its catalytic activity. However, we find that co-immobilization of the enzyme and the Fc-PEG co-substrate on GFLV does induce enzymatic enhancement, by promoting cooperativity between the two subunits of the homodimeric enzyme, via "synchronization" of their redox state. A decrease in inhibition of the enzyme by its substrate (glucose) is also observed.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Vianney Poignavent and Christophe Ritzenthaler have patent EP3596219A pending, which applies to the use of Nanobodies to functionalize GFLV.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE